NeuroGroup, Institute of Biomedical Technology, University of Tampere, Tampere, Finland; BioMediTech, Tampere, Finland; The Science Center of Pirkanmaa Hospital District, Tampere University Hospital, Tampere, Finland.
J Tissue Eng Regen Med. 2014 Mar;8(3):186-94. doi: 10.1002/term.1512. Epub 2012 May 18.
The future of tissue engineering applications for neuronal cells will require a supportive 3D matrix. This particular matrix should be soft, elastic and supportive for cell growth. In this study, we characterized the suitability of a 3D synthetic hydrogel matrix, PuraMatrix™, as a growth platform for human embryonic stem cell (hESC)-derived neural cells. The viability of the cells grown on top of, inside and under the hydrogel was monitored. The maturation and electrical activity of the neuronal networks inside the hydrogel were further characterized. We showed that cells stayed viable on the top of the PuraMatrix™ surface and growth of the neural cells and neural processes was good. Further, hESC-derived neurons, astrocytes and oligodendrocytes all grew, matured and migrated when cultured inside the hydrogel. Importantly, neuronal cells were able to form electrically active connections that were verified using microelectrode array. Thus, PuraMatrix is a good supportive growth matrix for human neural cells and may serve as a matrix for neuronal scaffolds in neural tissue engineering.
未来用于神经元细胞的组织工程应用将需要一个支持性的 3D 基质。这个特定的基质应该柔软、有弹性且支持细胞生长。在这项研究中，我们研究了 3D 合成水凝胶基质 PuraMatrix™作为人胚胎干细胞 (hESC) 衍生的神经细胞生长平台的适用性。监测了水凝胶上、内部和下方生长的细胞的活力。进一步表征了水凝胶内神经元网络的成熟和电活动。我们表明，细胞在 PuraMatrix™表面的顶部保持存活，并且神经细胞和神经突起的生长良好。此外，当在水凝胶内培养时，hESC 衍生的神经元、星形胶质细胞和少突胶质细胞都生长、成熟并迁移。重要的是，神经元细胞能够形成电活性连接，这可以使用微电极阵列进行验证。因此，PuraMatrix 是一种良好的人神经细胞支持性生长基质，可作为神经组织工程中神经元支架的基质。
