Soltzberg Joseph, Frischmann Sarah, van Heeckeren Christiaan, Brown Nicole, Caplan Arnold, Bonfield Tracey L
Hawken School, Gates Mills Ohio, USA.
Anal Quant Cytol Histol. 2011 Oct;33(5):245-52.
To develop a quantitative means to measure lung inflammation using the murine models of chronic asthma and cystic fibrosis (CF).
Translational-based medicine often utilizes animal models to study new and innovative therapeutics. In asthma and CF, the animal models focus on airway inflammation and remodeling. The asthma model is based on hypersensitivity-induced airway disease, whereas the CF model focuses on the inflammatory response to infection with Pseudomonas aeruginosa. Qualitative measures of inflammation and lung pathophysiology introduce significant variability and difficulty in interpreting interventional outcomes. The highly sensitive and reproducible quantitative computational program interfaced with Image Pro Microscopy to monitor changes in lung inflammation and lung pathophysiology. The software interfaces with image microscopy and automates the lung section review process.
Results from this program recapitulated data obtained by manual point counting of inflammation, bronchoalveolar lavage differential, and histology. The data show a low coefficient of variation and high reproducibility between slides and sections.
Utilization of this new microscopy program will enhance the quantitative means of establishing changes in lung structure and inflammation as a measure of therapeutic intervention with the ability of refining interpretation of in vivo models potentially short-circuiting translation into the clinical setting.
利用慢性哮喘和囊性纤维化（CF）小鼠模型开发一种测量肺部炎症的定量方法。
基于转化医学常常利用动物模型来研究新型和创新性治疗方法。在哮喘和CF中，动物模型聚焦于气道炎症和重塑。哮喘模型基于超敏反应诱导的气道疾病，而CF模型则侧重于对铜绿假单胞菌感染的炎症反应。炎症和肺病理生理学的定性测量在解释干预结果时引入了显著的变异性和困难。将高度敏感且可重复的定量计算程序与图像分析显微镜相结合，以监测肺部炎症和肺病理生理学的变化。该软件与图像显微镜相连，并使肺切片审查过程自动化。
该程序的结果概括了通过手动计数炎症、支气管肺泡灌洗差异和组织学获得的数据。数据显示，玻片和切片之间的变异系数低且重现性高。
使用这种新的显微镜程序将增强确定肺结构和炎症变化的定量方法，作为治疗干预的一种衡量标准，能够完善对体内模型的解释，可能缩短向临床环境转化的过程。
