Fu Hui, Hu Xiaojian, Ding Changming, Lin Shaobin
Institute of Environmental Health and Related Product Safety, China CDC, Beijing 100021, China.
Wei Sheng Yan Jiu. 2012 Mar;41(2):282-6.
To develop a method for determination of the Metronidazole, Chlortetracycline hydrochloride, Oxytetracycline dihydrate, Minocycline hydrochloride, Erythromycin and Tetracycline hydrochloride in disinfection products by high performance liquid chromatography tandem mass spectrometry(LC-MS/MS).
Samples were extracted by methanol and filtered through 0.45 microm PTFE membrane filter, then analyzed by LC-MS/MS using Waters Symmetry C18 (2.1 mm x 150 mm, 3.5 microm) column in positive ion scan mode. The mobile phase was 5 mmol/L ammonium acetate, methanol and acetonitrile.
The linear range was 0-2000 ng/ml and the correlation coefficients were more than 0.998, the average recoveries ranged from 74.7% to 114% with the relative standard deviations between 1.6%-20.2%. The method was successfully used to detect the content of antibiotics in 115 disinfection products.
The method is simple, sensitive, selective and suitable for the analysis of residual content of antibiotics in cream formulations of disinfection products.
建立高效液相色谱串联质谱法（LC-MS/MS）测定消毒产品中甲硝唑、盐酸金霉素、盐酸土霉素、盐酸米诺环素、红霉素及盐酸四环素的方法。
样品用甲醇提取，经0.45μm聚四氟乙烯（PTFE）滤膜过滤，采用Waters Symmetry C18（2.1mm×150mm，3.5μm）色谱柱，在正离子扫描模式下进行LC-MS/MS分析。流动相为5mmol/L醋酸铵、甲醇和乙腈。
线性范围为0 - 2000ng/ml，相关系数大于0.998，平均回收率为74.7% - 114%，相对标准偏差为1.6% - 20.2%。该方法成功用于检测115份消毒产品中的抗生素含量。
该方法简便、灵敏、选择性好，适用于消毒产品乳膏制剂中抗生素残留量的分析。
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