[人肺腺癌细胞系Ch-Huang-1的建立与鉴定]
[Establishment and characterization of a human lung adenocarcinoma cell line, Ch-Huang-1].
作者信息
Li Bailing, Zhong Keng, Jin Lei, Yuan Yang, Gong Dejun, Liu Xiaohong, Huang Shengdong
机构信息
Department of Cardiothoracic Surgery, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2012 May;15(5):267-70. doi: 10.3779/j.issn.1009-3419.2012.05.03.
BACKGROUND AND OBJECTIVE
Lung cancer is a major worldwide health problem. The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics.
METHODS
Lung adenocarcinoma specimens were freshly resected during surgery. The tissues were incubated in vitro and the cell line was named Ch-Huang-1. The biological characteristics of the cells were investigated by light microscopy, chromosome analysis, and transplantation experiment.
RESULTS
Light microscopy revealed that cells from the primary tumor, Ch-Huang-1 cell line, and transplanted tumor possessed the characteristics of a malignant glandular epithelial tumor. The cell growth curve, doubling time, and mitotic index were also observed in vitro. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 35-44 per cell. Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.
CONCLUSION
The characteristics of the established cell line suggest that it is a newly established human adenocarcinoma cell line.
背景与目的
肺癌是全球主要的健康问题。本研究旨在建立一种新型的中国人肺腺癌细胞系并检测其生物学特性。
方法
手术中新鲜切除肺腺癌标本。将组织进行体外培养，该细胞系命名为Ch-Huang-1。通过光学显微镜、染色体分析和移植实验研究细胞的生物学特性。
结果
光学显微镜显示，原发肿瘤细胞、Ch-Huang-1细胞系及移植瘤细胞具有恶性腺上皮肿瘤的特征。体外还观察了细胞生长曲线、倍增时间和有丝分裂指数。核染色体分析显示，该肿瘤为亚三倍体，每个细胞的染色体数众数为35 - 44条。通过异种移植在裸鼠皮下观察到肿瘤结节。
结论
所建立细胞系的特征表明它是一种新建立的人腺癌细胞系。
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