Wang Jing-Feng, Jin Min, Shen Zhi-Qiang, Qiu Zhi-Gang, Zhang Bin, Kong Qing-Xin, Li Jun-Wen
Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Huan Jing Ke Xue. 2011 Aug;32(8):2409-13.
To screen aerobic heterotrophic denitrifying bacteria from various samples and detect metabolites quantitatively during their denitrification process. The bacteria were screened by limit dilution and crossed separation. Nitrate and nitrite were determined using phenol disulfonic acid and N-(1-naphthyl)-ethylene diamine by ultraviolet spectrophotometry. The N2 was detected by gas chromatography. Six aerobic heterotrophic bacteria were isolated and YY-5 was demonstrated to be the greatest denitrifer. The degradation rate of its hydroxylamine oxidoreductase can reached to 70.9%. Above all, there is a good balance between the removal of total nitrogen and production of N2 in process. No N2O was produced and no nitrate or nitrite were accumulated. The high ability of aerobic heterotrophic bacterium YY-5 for denitrification was screened, which showed higher homology with Acinetobacter genus through 16S rRNA blast.
从各种样品中筛选好氧异养反硝化细菌，并在其反硝化过程中对代谢产物进行定量检测。通过有限稀释和交叉分离对细菌进行筛选。采用酚二磺酸法和N-(1-萘基)-乙二胺法，通过紫外分光光度法测定硝酸盐和亚硝酸盐。采用气相色谱法检测N₂。分离出6株好氧异养细菌，其中YY-5被证明是最强的反硝化菌。其羟胺氧化还原酶的降解率可达70.9%。最重要的是，在该过程中总氮去除和N₂产生之间存在良好的平衡。未产生N₂O，也没有硝酸盐或亚硝酸盐积累。筛选出了好氧异养细菌YY-5的高反硝化能力，通过16S rRNA比对显示其与不动杆菌属具有较高的同源性。
