Department of Environmental Engineering, Peking University, Beijing 100871, China.
Bioresour Technol. 2013 Jul;139:80-6. doi: 10.1016/j.biortech.2013.03.189. Epub 2013 Apr 6.
A psychrotrophic heterotrophic nitrifying-aerobic denitrifying bacterium was newly isolated and identified as Acinetobacter sp. with phenotypic and phylogenetic analysis. The strain possessed excellent tolerance to low temperature with 20°C as its optimum and 4°C as viable. Moreover, ammonium, nitrite and nitrate could be removed efficiently under low-temperature, solely aerobic conditions with little accumulation of intermediates. The average removal rate at 10°C reached as high as 3.03, 2.51 and 1.88 mg NL(-1)h(-1) for ammonium, nitrite and nitrate respectively. N2 was produced through heterotrophic nitrification and aerobic denitrification via nitrite but N2O was never detected in the whole process. Nitrogen balance analysis indicated that N2 and intracellular nitrogen were two major fates of the initial ammonium, accounting for 32.4 and 49.2%, respectively. Further aerated batch test demonstrated efficient removal of COD and TN from synthetic wastewater, which implied promising practical application of the present strain.
一种嗜冷异养硝化好氧反硝化细菌，经表型和系统发育分析，被新鉴定为不动杆菌属。该菌株对低温具有良好的耐受性，最适温度为 20°C，可在 4°C 下存活。此外，在低温、有氧条件下，铵、亚硝酸盐和硝酸盐可被有效去除，中间产物积累较少。在 10°C 时，铵、亚硝酸盐和硝酸盐的平均去除率分别高达 3.03、2.51 和 1.88 mg NL(-1)h(-1)。通过异养硝化和好氧反硝化途径亚硝酸盐产生 N2，但整个过程中从未检测到 N2O。氮平衡分析表明，N2 和细胞内氮是初始铵的两个主要归宿，分别占 32.4%和 49.2%。进一步的曝气批量试验表明，该菌株可有效去除合成废水中的 COD 和 TN，这意味着该菌株具有很好的实际应用前景。
