Lin Lin, An Jing, Zhou Qi-Xing
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Huan Jing Ke Xue. 2011 Aug;32(8):2430-5.
The ecotoxicological effects of tetracycline (TC) on the early growth and seedlings development of Chinese white cabbage were investigated using the pot-culture method. The results showed that the stem elongation of the cabbages was promoted, while the root elongation and fresh weight of the cabbages were inhibited by 1-100 mg/kg TC. After a 28-day exposure, the inhibition rate of root elongation was up to 36.0% and that of fresh weight was up to 34.6% stressed by 100 mg/kg TC. With the exposure time of TC prolonged, the content of chlorophyll in leaves of the cabbage seedlings stressed by 100 mg/kg TC was significantly lower than that treated by other concentrations of TC, with the inhibition rate of chlorophyll was 21.7% - 29.0%. After a 7-day exposure to TC, the content of soluble protein (SP) and malondialdehyde (MDA) in the cabbage seedlings changed insignificantly. With the exposure time of TC prolonged, the content of SP in the cabbage seedlings decreased significantly, while the content of MDA increased significantly. Moreover, the activity of SOD, POD and CAT in Chinese white cabbage seedlings was inhibited by 1-100 mg/kg TC. The inhibition rates of SOD, POD and CAT activity exposed to 100 mg/kg TC were 33.9% - 45.3%, 33.8% - 67.7% and 29.6% - 57.7%, respectively. It suggested that TC has ecotoxicological effects on Chinese white cabbage seedlings with a chronic exposure.
采用盆栽法研究了四环素(TC)对小白菜早期生长和幼苗发育的生态毒理效应。结果表明,1-100mg/kg的TC促进了白菜茎的伸长,而抑制了白菜根的伸长和鲜重。暴露28天后,100mg/kg的TC胁迫下根伸长的抑制率高达36.0%,鲜重的抑制率高达34.6%。随着TC暴露时间的延长,100mg/kg的TC胁迫下白菜幼苗叶片中叶绿素含量显著低于其他浓度处理,叶绿素抑制率为21.7%-29.0%。暴露于TC 7天后,白菜幼苗中可溶性蛋白(SP)和丙二醛(MDA)含量变化不显著。随着TC暴露时间的延长,白菜幼苗中SP含量显著降低,而MDA含量显著增加。此外,1-100mg/kg的TC抑制了小白菜幼苗中SOD、POD和CAT的活性。暴露于100mg/kg的TC下,SOD、POD和CAT活性的抑制率分别为33.9%-45.3%、33.8%-67.7%和29.6%-57.7%。这表明长期暴露下TC对小白菜幼苗具有生态毒理效应。