An Jing, Zhou Qixing, Sun Yuebing, Xu Zhiqiang
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
Chemosphere. 2009 Sep;76(10):1428-34. doi: 10.1016/j.chemosphere.2009.06.004. Epub 2009 Jul 24.
Biochemical responses of wheat (Triticum aestivum L.) seedlings stressed by two typical personal care products (PCPs)--triclosan (TCS) and galaxolide (HHCB)--were experimentally investigated to assess their ecological risks. The results showed that wheat shoot and root elongation was significantly inhibited by 50-250 mg L(-1) TCS and HHCB. Wheat roots were sensitive to TCS, while shoots were sensitive to HHCB. The median effect concentration (EC(50)) of TCS and HHCB based on the inhibition of their sensitive sites were 147.8 and 143.4 mg L(-1), respectively. Moreover, the damage of wheat seedlings treated by low concentration of TCS and HHCB during a long period cannot be neglected. After a 21-d exposure, 0.2-3.0mg L(-1) TCS and HHCB treatment caused the damage to the accumulation of chlorophyll (CHL), the synthesis of soluble protein (SP), and the activity of peroxidase (POD) and superoxide dismutases (SOD) in different degree. However, different changing trends of these physiological indexes treated by different PCPs were observed after 7-d to 14-d exposures, especially the activity of POD and SOD. The activity of POD and SOD in wheat leaves and roots decreased with an increase in the concentration of TCS and the exposure time. However, the enzyme activities in wheat leaves treated by 0.2-3.0mg L(-1) HHCB increased after a 14-d exposure, and with the prolongation of exposure time, the enzyme activities significantly decreased. The variations in these physiological indexes of wheat could be considered as good biomarkers of serious stress by TCS and HHCB in the environment.
通过实验研究了两种典型个人护理产品（PCPs）——三氯生（TCS）和佳乐麝香（HHCB）对小麦（Triticum aestivum L.）幼苗的生化响应，以评估它们的生态风险。结果表明，50 - 250 mg L(-1)的TCS和HHCB显著抑制了小麦地上部和根部的伸长。小麦根部对TCS敏感，而地上部对HHCB敏感。基于对其敏感部位的抑制作用，TCS和HHCB的半数效应浓度（EC(50)）分别为147.8和143.4 mg L(-1)。此外，低浓度的TCS和HHCB长期处理对小麦幼苗造成的损害不容忽视。经过21天的暴露，0.2 - 3.0 mg L(-1)的TCS和HHCB处理不同程度地导致了叶绿素（CHL）积累、可溶性蛋白（SP）合成以及过氧化物酶（POD）和超氧化物歧化酶（SOD）活性的损害。然而，在7 - 14天的暴露后，观察到不同PCPs处理的这些生理指标有不同的变化趋势，尤其是POD和SOD的活性。小麦叶片和根部中POD和SOD的活性随着TCS浓度和暴露时间的增加而降低。然而，0.2 - 3.0 mg L(-1)的HHCB处理14天后，小麦叶片中的酶活性增加，并且随着暴露时间的延长，酶活性显著降低。小麦这些生理指标的变化可被视为环境中TCS和HHCB严重胁迫的良好生物标志物。
