来自喀什驼绒藜的一种蛋白质合成起始抑制剂

An inhibitor of protein synthesis initiation from Alhagi kirgisorum S.

作者信息

Smailov S K, Mukhamedzhanov B G, Lee A V, Iskakov B K, Denisenko O N

机构信息

Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Alma-Ata, USSR.

出版信息

FEBS Lett. 1990 Nov 26;275(1-2):99-101. doi: 10.1016/0014-5793(90)81448-w.

DOI:10.1016/0014-5793(90)81448-w

PMID:2262008

Abstract

Polyproanthocyanidin--a plant phenolic compound from Alhagi kirgisorum S. effectively inhibited protein synthesis in rabbit reticulocyte and wheat germ cell-free systems. Poly-proanthocyanidin inhibited translation only at the level of initiation and not at the elongation level and aminoacylation of tRNA. The inhibitory effect of the phenolic compound is due to the blockage of the ternary complex formation of eIF-2 with GTP and initiator Met-tRNA.

摘要

聚原花青素——一种来自吉尔吉斯骆驼刺的植物酚类化合物，能有效抑制兔网织红细胞和小麦胚无细胞系统中的蛋白质合成。聚原花青素仅在起始水平而非延伸水平以及tRNA的氨酰化过程中抑制翻译。这种酚类化合物的抑制作用是由于eIF-2与GTP和起始甲硫氨酰-tRNA的三元复合物形成受阻。