Yigit A A, Cinar M, Yildirim E
Department of Physiology, Kırıkkale University, Kırıkkale, Turkey.
N Z Vet J. 2012 Sep;60(5):273-7. doi: 10.1080/00480169.2012.680190. Epub 2012 May 24.
To determine the effects of Cu and levamisole on concentrations of Cu and Fe in plasma and liver, and the effects of levamisole on lipid peroxidation induced by Cu intoxication in broiler chickens.
In a 2×4 factorial study, 80 one-day-old Ross PM3 broiler chicks were fed diets for 21 days containing either 8 mg/kg Cu (Low Cu) or 250 mg/kg Cu (High Cu) and were treated with 0 (L0), 4 (L4), 8 (L8) or 16 (L16) mg/kg bodyweight levamisole per day from Day 7 of the study, on three consecutive days in their drinking water. This treatment was repeated three times, at 3-day intervals. On Day 21, blood samples were collected from each bird for analysis of concentrations of Cu, Fe and malondialdehyde, and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase, catalase and glutathione peroxidase (GSH-Px). The birds were killed and liver samples collected for analysis of Cu and Fe.
Mean concentrations of Cu and Fe in plasma, and Cu in liver, were increased overall in the High Cu groups compared with the Low Cu groups (p<0.001). Compared with the L0 treatment group on the High Cu diet, treatments L4, L8 and L16 decreased concentrations of Cu in plasma, and L8 and L16 increased concentration of Cu in liver (p<0.05). Mean activities of AST and ALT were increased in untreated birds (L0) fed the High compared with Low Cu diets (p<0.01). In birds receiving the High Cu diet, treatments L4 and L8 decreased activities of AST, and L4 and L16 decreased activity of ALT, compared with L0 (p<0.05). The High Cu diet induced an oxidative stress characterised by increased mean concentrations of malondialdehyde and decreased activities of superoxide dismutase, catalase and GSH-Px (p<0.001). Concentration of malondialdehyde, and activities of superoxide dismutase and catalase were not changed following levamisole treatment in birds on the High Cu diet, and activity of GSH-Px was decreased by the L4 and L8 treatments compared with the L0 group.
The results of the study suggest that treatment with levamisole might alleviate the harmful effects of Cu on the liver, as demonstrated by decreased activities of AST and ALT induced by a diet containing 250 mg/kg Cu.
确定铜和左旋咪唑对肉鸡血浆和肝脏中铜和铁浓度的影响，以及左旋咪唑对铜中毒诱导的脂质过氧化的影响。
在一项2×4析因研究中，80只1日龄罗斯PM3肉鸡雏鸡被喂食含8毫克/千克铜（低铜）或250毫克/千克铜（高铜）的日粮21天，并从研究第7天开始，连续三天在其饮水中每天用0（L0）、4（L4）、8（L8）或16（L16）毫克/千克体重的左旋咪唑进行处理。这种处理每隔3天重复3次。在第21天，从每只鸡采集血样，分析铜、铁和丙二醛的浓度，以及天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-Px）的活性。处死鸡并采集肝脏样本分析铜和铁。
与低铜组相比，高铜组血浆中铜和铁的平均浓度以及肝脏中铜的平均浓度总体上有所增加（p<0.001）。与高铜日粮的L0处理组相比，L4、L8和L16处理降低了血浆中铜的浓度，L8和L16增加了肝脏中铜的浓度（p<0.05）。与低铜日粮相比，未处理的高铜日粮喂养的鸡（L0）中AST和ALT的平均活性增加（p<0.01）。在接受高铜日粮的鸡中，与L0相比，L4和L8处理降低了AST的活性，L4和L16处理降低了ALT的活性（p<0.05）。高铜日粮诱导了氧化应激，其特征是丙二醛平均浓度增加，超氧化物歧化酶、过氧化氢酶和GSH-Px的活性降低（p<0.001）。高铜日粮鸡经左旋咪唑处理后，丙二醛浓度、超氧化物歧化酶和过氧化氢酶活性未发生变化，与L0组相比，L4和L8处理降低了GSH-Px的活性。
研究结果表明，左旋咪唑处理可能减轻铜对肝脏的有害影响，如含250毫克/千克铜日粮诱导的AST和ALT活性降低所示。
