大肠杆菌素E7和E8可降解敏感细菌中的DNA。
Colicins E7 and E8 degrade DNA in sensitive bacteria.
作者信息
Smarda J, Smarda J, Vrbická Z
机构信息
Department of Biology, Medical Faculty, Masaryk University, Brno, Czechoslovakia.
出版信息
Folia Microbiol (Praha). 1990;35(4):348-52. doi: 10.1007/BF02821286.
The primary target of colicin E7 in sensitive bacteria are their DNA molecules. In agarose gel electrophoresis of lysates of cells treated with colicin E7, both chromosomal and plasmid DNA bands disappear, in direct relation to E7 concentration and to the duration of treatment. DNA degradation is followed by a cessation of DNA synthesis. In E7-immune bacteria, no damage to DNA due to colicin E7 occurs. The mode of action of colicin E7 thus appears to be equal to that of colicin E2. Also, colicin E8 causes a distinct damage to chromosomal and plasmid DNA in sensitive, but not in immune bacteria. None of the colicins E1, E3, E4, E5, E6 or E9 has any influence on bacterial DNA.
大肠杆菌素E7在敏感细菌中的主要靶标是它们的DNA分子。在用大肠杆菌素E7处理的细胞裂解物的琼脂糖凝胶电泳中，染色体和质粒DNA条带均消失，这与E7浓度和处理持续时间直接相关。DNA降解之后是DNA合成的停止。在对大肠杆菌素E7免疫的细菌中，不会因大肠杆菌素E7而对DNA造成损伤。因此，大肠杆菌素E7的作用方式似乎与大肠杆菌素E2相同。此外，大肠杆菌素E8会对敏感细菌（而非免疫细菌）的染色体和质粒DNA造成明显损伤。大肠杆菌素E1、E3、E4、E5、E6或E9对细菌DNA均无任何影响。
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