大肠杆菌中大肠杆菌素质粒E1、E2和E3表现出的不相容性。
Incompatibility exhibited by colicin plasmids E1, E2, and E3 in Escherichia coli.
作者信息
Inselburg J
出版信息
J Bacteriol. 1974 Aug;119(2):478-83. doi: 10.1128/jb.119.2.478-483.1974.
Escherichia coli strains were made multiply colicinogenic for the colicin plasmids E1, E2, or E3 (Col E1, Col E2, or Col E3, respectively) by both a deoxyribonucleic acid transformation system and bacterial conjugation. The multiply colicinogenic bacteria constructed exhibited an immunity to the colicins produced by all the plasmids they carried and also produced colicins corresponding to all the plasmids they carried. An incompatibility was observed among the plasmids. In doubly colicinogenic cells where the presence of two plasmids was established, Col E2 was lost more frequently than Col E3. In triply colicinogenic cells, Col E1, Col E2, and Col E3 were lost, with Col E3 being lost least frequently. A significant reduction in the acquisition of a conjugationally transferred Col E1 plasmid by cells colicinogenic for Col E1 was demonstrated.
通过脱氧核糖核酸转化系统和细菌接合作用，使大肠杆菌菌株对大肠杆菌素质粒E1、E2或E3（分别为Col E1、Col E2或Col E3）具有多重产大肠杆菌素能力。构建的多重产大肠杆菌素细菌对它们所携带的所有质粒产生的大肠杆菌素具有免疫性，并且还产生与它们所携带的所有质粒相对应的大肠杆菌素。观察到这些质粒之间存在不相容性。在已确定存在两种质粒的双重产大肠杆菌素细胞中，Col E2比Col E3更频繁地丢失。在三重产大肠杆菌素细胞中，Col E1、Col E2和Col E3都会丢失，其中Col E3丢失的频率最低。结果表明，对Col E1产大肠杆菌素的细胞通过接合转移获得Col E1质粒的能力显著降低。
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