Rekesh A N, Seliverstova S A, Nikitin A N, Khmel' I A
Mol Biol (Mosk). 1982 Jan-Feb;16(1):177-82.
Plasmid proteins with m. w. of 14 000--15 000 were chromatographically isolated from lysates of E. coli cells containing plasmids ColK and ColE1. These proteins can reversibly inhibit the killing activity of K and E1 colicine and appear to be the immunity proteins of ColK and ColE1 plasmids and provide the defence of colicinogenic cells from their own colicin. The immunity protein of the ColK plasmid acts both upon K colicin and E1 colicin and vice versa. Unlike E2, E3 colicins or CloDF13 cloacin, K and E1 colicins are extracted from cells in a free state and not in a complex with the immunity protein.
从含有质粒ColK和ColE1的大肠杆菌细胞裂解物中通过色谱法分离出分子量为14000 - 15000的质粒蛋白。这些蛋白可可逆地抑制K和E1大肠杆菌素的杀伤活性，似乎是ColK和ColE1质粒的免疫蛋白，并为产大肠杆菌素的细胞提供针对自身大肠杆菌素的防御。ColK质粒的免疫蛋白对K大肠杆菌素和E1大肠杆菌素均起作用，反之亦然。与E2、E3大肠杆菌素或CloDF13梭链孢酸不同，K和E1大肠杆菌素以游离状态从细胞中提取，而非与免疫蛋白形成复合物。
