Universidade do Vale do Itajaí - UNIVALI, Centro de Ciências Tecnológicas da Terra e do Mar - CTTMar, Cx.P. 360, Itajaí, SC 88302-202, Brazil.
Chemosphere. 2012 Sep;89(1):102-7. doi: 10.1016/j.chemosphere.2012.04.033. Epub 2012 May 22.
Since the development of the TIE (Toxicity Identification and Evaluation) in 1988 it has been assumed that the capacity of EDTA and sodium thiosulfate to complex some metals, and thus remove their toxicity, can be applied to both freshwater and seawater ecotoxicological tests and the results subsequently interpreted. However, it is now known that there is a wide variability in the extent of this complexation. In this context, the removal of toxicity caused by the presence of Hg(2+), Cd(2+), Cu(2+), Cr(6+), Zn(2+), Ni(2+), Pb(2+), Ag(1+) and Se(2+), through metal complexation by EDTA and sodium thiosulfate, in relation to the performance of embryo-larval tests with the sea urchin Arbacia lixula was investigated. It was observed that EDTA was capable of removing the toxicity of Pb(2+), Zn(2+) and Cu(2+) while sodium thiosulfate only reduced the toxicity of Ag(1+). Compared to the complexation observed in freshwater ecotoxicological tests, the complexing agents used in this study (EDTA and sodium thiosulfate) have a lower capacity to complex metals in the marine ecotoxicological test with A. lixula.
自 1988 年开发 TIE（毒性识别和评估）以来，人们一直认为 EDTA 和硫代硫酸钠与一些金属形成配合物的能力，从而去除其毒性，可以应用于淡水和海水生态毒理学测试，并随后对结果进行解释。然而，现在已知这种配合的程度存在很大的可变性。在这种情况下，通过 EDTA 和硫代硫酸钠与 Hg(2+)、Cd(2+)、Cu(2+)、Cr(6+)、Zn(2+)、Ni(2+)、Pb(2+)、Ag(1+)和 Se(2+)等金属形成配合物，研究了其对海胆 Arbacia lixula 胚胎幼虫试验毒性的去除效果。结果表明，EDTA 能够去除 Pb(2+)、Zn(2+)和 Cu(2+)的毒性，而硫代硫酸钠仅降低了 Ag(1+)的毒性。与淡水生态毒理学测试中观察到的配合相比，本研究中使用的配合剂（EDTA 和硫代硫酸钠）在 A. lixula 的海洋生态毒理学测试中对金属的配合能力较低。
