Hammer V A, Gietzen D W, Sworts V D, Beverly J L, Rogers Q R
Department of Physiological Sciences, School of Veterinary Medicine, University of California, Davis 95616.
J Nutr. 1990 Dec;120(12):1617-23. doi: 10.1093/jn/120.12.1617.
The role of adrenal function in the anorectic response and adaptation of rats to a diet with an isoleucine (Ile) imbalance was investigated. In the first of four experiments, rats were fed a mildly Ile-imbalanced diet after treatment with metyrapone, and inhibitor of glucocorticoid synthesis. In two separate experiments, rats were presented with either a mildly or severely Ile-imbalanced diet (4.93 and 9.86% imbalanced amino acid mixture, respectively) after bilateral adrenalectomy. Finally, the effects of ICS 205-930, a serotonin-3 receptor antagonist, on the intake of mildly Ile-imbalanced diet were tested in adrenalectomized animals. In each experiment a 2 X 2 factorial design was used. Neither metyrapone nor adrenalectomy altered the initial depression in the intake of an imbalanced diet. The adaptation phase in the response of adrenalectomized rats fed a mildly Ile-imbalanced diet was not different from that of controls, but adrenalectomized rats fed severely Ile-imbalanced diets were unable to adapt. Adrenalectomy did not alter the anti-anoretic activity of ICS 205-930 in this model. These results suggest that adrenal hormones are not necessary for the initial anoretic response or adaptation of rats to an Ile-imbalanced diet, nor are they implicated in the anti-anorectic effect of serotonin-3 blockade.
研究了肾上腺功能在大鼠厌食反应以及对异亮氨酸（Ile）失衡饮食的适应过程中的作用。在四个实验的第一个实验中，用糖皮质激素合成抑制剂甲吡酮处理大鼠后，给它们喂食轻度Ile失衡的饮食。在另外两个独立实验中，双侧肾上腺切除术后，给大鼠分别喂食轻度或重度Ile失衡的饮食（不平衡氨基酸混合物分别为4.93%和9.86%）。最后，在肾上腺切除的动物中测试了5-羟色胺-3受体拮抗剂ICS 205-930对轻度Ile失衡饮食摄入量的影响。每个实验都采用了2×2析因设计。甲吡酮和肾上腺切除术均未改变不平衡饮食摄入量最初的下降情况。喂食轻度Ile失衡饮食的肾上腺切除大鼠的适应阶段与对照组没有差异，但喂食重度Ile失衡饮食的肾上腺切除大鼠无法适应。在该模型中，肾上腺切除术未改变ICS 205-930的抗厌食活性。这些结果表明，肾上腺激素对于大鼠对Ile失衡饮食的最初厌食反应或适应并非必需，它们也与5-羟色胺-3阻断的抗厌食作用无关。
