Microbiology Division, Central Leather Research Institute, (CSIR, New Delhi), Adyar, Chennai, Tamil Nadu.
Int J Radiat Biol. 2012 Aug;88(8):575-82. doi: 10.3109/09553002.2012.697644. Epub 2012 Jun 26.
The present study investigates the inhibition of Ultraviolet B (UVB, 290-320 nm) radiation-induced oxidative damage in peripheral blood human lymphocytes by embelin extracted from Embelia ribes.
Embelin was extracted, purified and characterized. Prior to inhibitory assessment, a maximum concentration of embelin that was non-toxic was determined. Six experimental groups, including respective controls were made to assess the inhibitory effect of embelin for the selected concentrations of 10 and 20 μg/ml. For the experimental groups; lymphocytes (1 × 10(6) cells) were pre-treated with the chosen concentration of embelin for a period of 60 min and then exposed to UVB for 30 min. UVB radiation inhibitory effect of embelin assessed by measuring antioxidant and lipid peroxidation levels, deoxyribonucleic acid (DNA) damage, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) at scheduled time points after irradiation.
Pre-treatment of lymphocytes with embelin prevents UVB-induced oxidative damage. An increase in antioxidant levels in irradiated cells in the presence of embelin and UV absorbance of embelin could be the reason for the decrease in lipid peroxidation level and prevention of DNA damage by UVB radiation.
Embelin prevents oxidative stress induced by UVB irradiation via its antioxidant property.
本研究旨在探讨从黑果枸杞中提取的杨梅素对人外周血淋巴细胞中紫外线 B（UVB，290-320nm）辐射诱导的氧化损伤的抑制作用。
杨梅素进行了提取、纯化和鉴定。在进行抑制评估之前，确定了非毒性的杨梅素最大浓度。制作了六个实验组，包括各自的对照组，以评估 10 和 20μg/ml 选定浓度的杨梅素的抑制作用。对于实验组；将选定浓度的杨梅素预处理淋巴细胞（1×10(6)个细胞）60 分钟，然后暴露于 UVB 30 分钟。通过在照射后预定时间点测量抗氧化和脂质过氧化水平、脱氧核糖核酸（DNA）损伤、活性氧（ROS）和线粒体膜电位（MMP），评估杨梅素对 UVB 的抑制作用。
杨梅素预处理可预防 UVB 诱导的氧化损伤。在存在杨梅素和 UV 吸收的情况下，辐照细胞中抗氧化剂水平的增加可能是脂质过氧化水平降低和预防 DNA 损伤的原因由 UVB 辐射引起。
杨梅素通过其抗氧化特性防止 UVB 照射引起的氧化应激。
