Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
J Ethnopharmacol. 2010 Feb 17;127(3):596-601. doi: 10.1016/j.jep.2009.12.019. Epub 2009 Dec 22.
Calendula officinalis flowers have long been employed time in folk therapy, and more than 35 properties have been attributed to decoctions and tinctures from the flowers. The main uses are as remedies for burns (including sunburns), bruises and cutaneous and internal inflammatory diseases of several origins. The recommended doses are a function both of the type and severity of the condition to be treated and the individual condition of each patient. Therefore, the present study investigated the potential use of Calendula officinalis extract to prevent UV irradiation-induced oxidative stress in skin.
Firstly, the physico-chemical composition of marigold extract (ME) (hydroalcoholic extract) was assessed and the in vitro antioxidant efficacy was determined using different methodologies. Secondly, the cytotoxicity was evaluated in L929 and HepG2 cells with the MTT assay. Finally, the in vivo protective effect of ME against UVB-induced oxidative stress in the skin of hairless mice was evaluated by determining reduced glutathione (GSH) levels and monitoring the secretion/activity of metalloproteinases.
The polyphenol, flavonoid, rutin and narcissin contents found in ME were 28.6 mg/g, 18.8 mg/g, 1.6 mg/g and 12.2mg/g, respectively and evaluation of the in vitro antioxidant activity demonstrated a dose-dependent effect of ME against different radicals. Cytoxicity experiments demonstrated that ME was not cytotoxic for L929 and HepG2 cells at concentrations less than or equal to of 15 mg/mL. However, concentrations greater than or equal to 30 mg/mL, toxic effects were observed. Finally, oral treatment of hairless mice with 150 and 300 mg/kg of ME maintained GSH levels close to non-irradiated control mice. In addition, this extract affects the activity/secretion of matrix metalloproteinases 2 and 9 (MMP-2 and -9) stimulated by exposure to UVB irradiation. However, additional studies are required to have a complete understanding of the protective effects of ME for skin.
金盏花在民间疗法中已有很长的应用历史，其花的煎剂和酊剂被认为具有 35 种以上的特性。主要用途是治疗烧伤（包括晒伤）、瘀伤以及多种来源的皮肤和内部炎症性疾病。建议剂量取决于要治疗的病症类型和严重程度以及每位患者的个体状况。因此，本研究调查了金盏花提取物预防皮肤紫外线照射诱导的氧化应激的潜在用途。
首先，评估了金盏花提取物（ME）（水醇提取物）的理化组成，并使用不同方法学评估了其体外抗氧化功效。其次，通过 MTT 测定法评估了 ME 在 L929 和 HepG2 细胞中的细胞毒性。最后，通过测定皮肤中还原型谷胱甘肽（GSH）水平和监测金属蛋白酶的分泌/活性，评估 ME 对无毛小鼠皮肤中 UVB 诱导的氧化应激的体内保护作用。
ME 中的多酚、类黄酮、芦丁和水仙苷含量分别为 28.6mg/g、18.8mg/g、1.6mg/g 和 12.2mg/g，体外抗氧化活性评估表明 ME 对不同自由基具有剂量依赖性作用。细胞毒性实验表明，ME 在浓度低于或等于 15mg/mL 时对 L929 和 HepG2 细胞无细胞毒性。然而，当浓度大于或等于 30mg/mL 时，观察到毒性作用。最后，用 150 和 300mg/kg 的 ME 对无毛小鼠进行口服治疗，使 GSH 水平接近未照射对照小鼠。此外，该提取物影响暴露于 UVB 照射刺激的基质金属蛋白酶 2 和 9（MMP-2 和 MMP-9）的活性/分泌。然而，需要进一步的研究来全面了解 ME 对皮肤的保护作用。
