Paunesku T, Stevanović M, Radosavljević D, Drmanac R, Crkvenjakov R
Institute for Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Mol Biol Evol. 1990 Sep;7(5):407-22. doi: 10.1093/oxfordjournals.molbev.a040616.
We have reported in rat three adult beta-gene haplotypes containing either five or three genes. Detailed sequence analysis reveals that the leftmost gene is the major gene and that at the opposite end downstream lies the minor gene. All of the genes lying between them are minor-major hybrids indicating their origin by unequal crossing-over. In two haplotypes beta-globin genes were found with an L1(1) element inserted directly into IVS2. The described results allow the formulation of a pathway of mutational events leading from the ancient two-beta-gene rodent ancestor through a three-gene haplotype to five-gene haplotypes, one of which is postulated to have arisen in common laboratory strains since their capture in the wild.
我们已经报道了大鼠中三种包含五个或三个基因的成年β基因单倍型。详细的序列分析表明，最左边的基因是主要基因，而在其相对的下游末端是次要基因。位于它们之间的所有基因都是次要-主要杂种，表明它们起源于不等交换。在两种单倍型中，发现β-珠蛋白基因有一个L1(1)元件直接插入到IVS2中。上述结果使得能够构建一个突变事件途径，该途径从古老的双β基因啮齿动物祖先开始，经过一个三基因单倍型到五基因单倍型，其中一种单倍型被推测自野生捕获后在常见实验室品系中出现。
