Stevanović M, Paunesku T, Radosavljević D, Drmanac R, Crkvenjakov R
Genetic Engineering Center, Belgrade, Yugoslavia.
Gene. 1989 Jun 30;79(1):139-50. doi: 10.1016/0378-1119(89)90099-1.
The genomic organization of three haplotypes of beta-globin genes was determined to resolve the question of the number of those genes in rat. Haplotype a, found in inbred strain DA, has three genes or pseudogenes, while haplotypes b, found in AO, Y5 and Wistar strains, and c, found in Wistar strain, have five genes or pseudogenes each. In haplotypes b and c, the first gene is of beta major type and the remaining four are of beta minor type. Partial sequencing of six out of 13 genes shows that duplications of beta minor genes are causing polymorphism in a number of genes. Also, in haplotype b two beta minor genes have a 6.5-kb intron 2, while in haplotype c only one beta minor gene contains such a large intron 2. The three structurally different haplotypes described are not interconvertible by single recombination events. The results indicate that the rat has the highest number of adult beta-globin genes found in mammals so far.
为了解决大鼠中β-珠蛋白基因数量的问题，我们确定了三种β-珠蛋白基因单倍型的基因组结构。在近交系DA中发现的单倍型a有三个基因或假基因，而在AO、Y5和Wistar品系中发现的单倍型b以及在Wistar品系中发现的单倍型c各有五个基因或假基因。在单倍型b和c中，第一个基因是β主要类型，其余四个是β次要类型。对13个基因中的6个进行部分测序表明，β次要基因的重复导致了许多基因的多态性。此外，在单倍型b中，两个β次要基因有一个6.5 kb的内含子2，而在单倍型c中，只有一个β次要基因含有如此大的内含子2。所描述的三种结构不同的单倍型不能通过单一重组事件相互转换。结果表明，大鼠拥有迄今为止在哺乳动物中发现的数量最多的成年β-珠蛋白基因。
