Vorontsova N I, Evgrafov O V, Makarov V B
Mol Gen Mikrobiol Virusol. 1990 Oct(10):31-2.
Efficiency of transformation by a number of vectors with the different selective markers of a set of cell lines has been studied for three different methods based on using calcium phosphate, polybrene, or electroporation. Electroporation is shown to be the most efficient one. Using this method with the system rat2k-cells-pAGO vector we have obtained the frequencies of transformation up to 2-3.10(-3). We suggest to use this system as a model for investigation of homologous recombination in the framework of the gene therapy project.
针对基于使用磷酸钙、多聚溴乙锭或电穿孔的三种不同方法，研究了一系列带有不同选择标记的载体对一组细胞系的转化效率。结果表明电穿孔是最有效的方法。使用大鼠2k细胞-pAGO载体系统的这种方法，我们获得了高达2 - 3×10⁻³ 的转化频率。我们建议将该系统用作基因治疗项目框架内同源重组研究的模型。
