Abe A, Miyanohara A, Friedmann T
Department of Pediatrics, Center for Molecular Genetics, University of California, San Diego 92093-0634, USA.
Gene Ther. 1998 May;5(5):708-11. doi: 10.1038/sj.gt.3300652.
Lipofection involves the introduction of foreign genetic information into mammalian cells through the use of lipophilic reagents that enhance cellular uptake of polynucleotides. Despite the use of currently optimized lipofection conditions, including the use of serum-depleted media, the efficiency of gene transfer is often low. We show here that, in a variety of cell lines, polybrene markedly enhances the efficiency of lipofection under standardized conditions and also compensates the serum-mediated inhibition of lipofection. Although the degree of the polybrene effect depends on the nature of the cell line, these results indicate that individually optimized concentrations of polybrene can be useful for increasing the efficiency of lipofectin-mediated gene transfer in vitro.
脂质转染法是指通过使用亲脂性试剂来增强细胞对多核苷酸的摄取，从而将外源遗传信息导入哺乳动物细胞。尽管目前使用了优化的脂质转染条件，包括使用无血清培养基，但基因转移效率往往较低。我们在此表明，在多种细胞系中，在标准化条件下，聚凝胺能显著提高脂质转染的效率，并且还能补偿血清对脂质转染的抑制作用。尽管聚凝胺作用的程度取决于细胞系的性质，但这些结果表明，单独优化聚凝胺的浓度有助于提高体外脂质体介导的基因转移效率。
