Morello Samantha, Southwood Louise L, Engiles Julie, Slack Joann, Crack Aaron, Springate Christopher M K
Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA, USA.
Vet Surg. 2012 Jul;41(5):568-81. doi: 10.1111/j.1532-950X.2012.00951.x. Epub 2012 May 25.
To evaluate the effect of PERIDAN™ Concentrate on clinical findings, infection, and tissue healing in adult horses undergoing celiotomy and jejunojejunostomy.
Block randomized blinded experimental in vivo study.
Adult horses (n = 12).
Horses had jejunojejunostomy at 2 sites and were administered 5 L of diluted PERIDAN™ Concentrate (6 horses) or Lactated Ringer's Injection (LRS) control intraperitoneally (6 horses) before body wall closure. Postoperative monitoring comprised physical examinations, serial hematology, coagulation and chemistry panels, and ultrasonographic examination. Horses were euthanatized 10 days postoperatively. Anastomoses and linea alba incisions were tested for mechanical strength; and tissue healing, inflammation, and infection were assessed by histological evaluation. Data were analyzed using a mixed model ANOVA. Level of significance was P < .05.
No physical examination differences were observed between groups. Statistically significant differences were observed in leukocyte and neutrophil counts, prothrombin time, antithrombin III activity, intestinal bursting pressures, and histologic healing grade in the mid region of the linea alba. These differences were minimal, and of no observable clinical significance. Other blood variable and histologic differences between groups were not significant.
PERIDAN™ Concentrate was safely administered intraperitoneally to healthy horses undergoing celiotomy and anastomosis.
评估PERIDAN™浓缩液对接受剖腹术和空肠空肠吻合术的成年马匹的临床症状、感染及组织愈合的影响。
体内区组随机双盲实验研究。
成年马（n = 12）。
马匹在两个部位进行空肠空肠吻合术，在关闭体壁前，给6匹马腹腔内注射5升稀释的PERIDAN™浓缩液，另外6匹马注射乳酸林格氏注射液（LRS）作为对照。术后监测包括体格检查、系列血液学检查、凝血和生化指标检测以及超声检查。术后10天对马匹实施安乐死。测试吻合口和白线切口的机械强度；通过组织学评估来评估组织愈合、炎症和感染情况。采用混合模型方差分析对数据进行分析。显著性水平为P < 0.05。
两组之间在体格检查方面未观察到差异。在白细胞和中性粒细胞计数、凝血酶原时间、抗凝血酶III活性、肠破裂压力以及白线中部的组织学愈合等级方面观察到了具有统计学意义的差异。这些差异极小，无明显临床意义。两组之间的其他血液变量和组织学差异不显著。
PERIDAN™浓缩液腹腔内注射给药于接受剖腹术和吻合术的健康马匹是安全的。
