Department of Pathophysiology, University of Zagreb, Zagreb, Croatia.
J Proteomics. 2012 Jul 19;75(14):4412-28. doi: 10.1016/j.jprot.2012.05.021. Epub 2012 May 23.
Cow serum proteome was evaluated by three different complementary approaches in the control group, subclinical and clinical mastitis in order to possibly find differential protein expression useful for a better understanding of the pathophysiology of mastitis as well as for an early diagnosis of the disease. The systemic inflammatory and oxidative stress response in cows with subclinical and clinical mastitis were observed. The collected evidence shows a differential protein expression of serpin A3-1, vitronectin-like protein and complement factor H in subclinical mastitis in comparison with the control. It was also found a differential protein expression of inter-alpha-trypsin inhibitor heavy chain H4, serpin A3-1, C4b-binding protein alpha chain, haptoglobin and apolipoprotein A-I in clinical mastitis compared to the control. Among the inflammatory proteins up-regulated in clinical mastitis, vitronectin is over-expressed in both subclinical and clinical mastitis indicating a strong bacterial infection. This suggests vitronectin as an important mediator in the pathogenesis of the onset of mastitis as well as a valuable marker for diagnosis of the subclinical form of the disease. Obtained data could be useful for the detection of mastitis during the subclinical phase and for a better comprehension of the pathophysiological mechanisms involved in the onset of the disease.
采用三种不同的互补方法评估了牛血清蛋白质组，包括对照组、亚临床型乳腺炎和临床型乳腺炎，以寻找可能有助于更好地理解乳腺炎病理生理学以及进行疾病早期诊断的差异蛋白表达。观察了亚临床型和临床型乳腺炎奶牛的全身炎症和氧化应激反应。研究结果表明，与对照组相比，亚临床型乳腺炎中丝氨酸蛋白酶抑制剂 A3-1、类 vitronectin 蛋白和补体因子 H 的蛋白表达存在差异。与对照组相比，临床型乳腺炎中还发现了 α1-胰蛋白酶抑制剂重链 H4、丝氨酸蛋白酶抑制剂 A3-1、C4b 结合蛋白 α 链、触珠蛋白和载脂蛋白 A-I 的蛋白表达存在差异。在临床型乳腺炎中上调的炎症蛋白中，vitronectin 在亚临床型和临床型乳腺炎中均过度表达，表明存在强烈的细菌感染。这表明 vitronectin 是乳腺炎发病机制中的一个重要介质，也是诊断亚临床型乳腺炎的有价值的标志物。获得的数据可能有助于在亚临床阶段检测乳腺炎，并更好地理解疾病发病机制中涉及的病理生理机制。
