嗜热四膜虫B1868VII株系染色体外rDNA分子的完整序列
Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII.
作者信息
Engberg J, Nielsen H
机构信息
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
出版信息
Nucleic Acids Res. 1990 Dec 11;18(23):6915-9. doi: 10.1093/nar/18.23.6915.
Abstract
The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.
摘要
用于四膜虫转化的重组DNA载体的最新进展，再加上改进的显微注射技术，应该会引发对这种生物体的新兴趣。特别是，重组DNA本身构成了一个用于生化研究的有吸引力的系统。重组DNA被扩增到总DNA的2%的水平，并以染色体外分子的形式存在。此外，重组DNA在序列上是同质的，因为它是在有性重组过程中从单个基因衍生而来的。为了便于对该分子进行研究，我们在此报告了先前发表的序列信息汇编以及新的序列数据，这些数据完成了21 kb重组DNA分子的完整序列。
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本文引用的文献
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Single extrachromosomal ribosomal RNA gene copies are synthesized during amplification of the rDNA in Tetrahymena.在四膜虫rDNA扩增过程中会合成单个染色体外核糖体RNA基因拷贝。
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Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.嗜热四膜虫的染色体外核糖体脱氧核糖核酸的两个独立区域能够使质粒在酿酒酵母中自主复制。
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