不同四膜虫物种中，染色体外rDNA回文序列中心附近一个38 bp区域具有高度的序列保守性。
Strong sequence conservation of a 38 bp region near the center of the extrachromosomal rDNA palindrome in different Tetrahymena species.
作者信息
Engberg J
出版信息
Nucleic Acids Res. 1983 Jul 25;11(14):4939-46. doi: 10.1093/nar/11.14.4939.
Abstract
The restriction-endonuclease map and the nucleotide sequence of the central region in the extrachromosomal rDNA palindrome of two micronuclear and one a-micronucleate species of Tetrahymena has been determined. The sequence data show that the different species investigated have a 24 or 26 nucleotide sequence region at the very center of the rDNA molecule which is non-palindromic. Comparison of the present sequence data with the published data of another micronucleate species reveal that a segment of 38 base pairs just outside the non-palindromic center is highly conserved in all the different species, while the rest of the central region show little sequence homology. The relevance of this conserved region to the amplification process of the rDNA molecule is discussed.
摘要
已确定了嗜热四膜虫的两个小核和一个α小核物种的染色体外rDNA回文序列中央区域的限制性内切酶图谱和核苷酸序列。序列数据表明，所研究的不同物种在rDNA分子的正中心有一个24或26个核苷酸的序列区域，该区域是非回文的。将目前的序列数据与另一个小核物种的已发表数据进行比较，发现在所有不同物种中，非回文中心外的一段38个碱基对的片段高度保守，而中央区域的其余部分则几乎没有序列同源性。讨论了该保守区域与rDNA分子扩增过程的相关性。
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