Kumar Megha, Chapman Susan C
Clemson University, Biological Sciences, Long Hall, Clemson, SC 29634, USA.
Gene Expr Patterns. 2012 Aug-Sep;12(7-8):245-53. doi: 10.1016/j.gep.2012.05.002. Epub 2012 May 24.
FGFs with similar sequences can play different roles depending on the model organisms examined. Determining these roles requires knowledge of spatio-temporal Fgf gene expression patterns. In this study, we report the cloning of chick Fgf5, 6 and 7, and examine their gene expression patterns by whole mount in situ hybridization. We show that Fgf5's spatio-temporally restricted expression pattern indicates a potentially novel role during inner ear development. Fgf6 and Fgf7, although belonging to different subfamilies with diverged sequences, are expressed in similar patterns within the mesoderm. Alignment of protein sequences and phylogenetic analysis demonstrate that FGF5 and FGF6 are highly conserved between chick, human, mouse and zebrafish. FGF7 is similarly conserved except for the zebrafish, which has considerably diverged.
具有相似序列的成纤维细胞生长因子（FGFs）根据所研究的模式生物不同而发挥不同作用。确定这些作用需要了解Fgf基因的时空表达模式。在本研究中，我们报告了鸡Fgf5、6和7的克隆，并通过整体原位杂交检测了它们的基因表达模式。我们发现Fgf5的时空受限表达模式表明其在内耳发育过程中可能具有新的作用。Fgf6和Fgf7虽然属于不同亚家族且序列存在差异，但在中胚层内以相似模式表达。蛋白质序列比对和系统发育分析表明，FGF5和FGF6在鸡、人、小鼠和斑马鱼之间高度保守。FGF7同样保守，只是斑马鱼的FGF7序列有较大差异。
