利用线粒体 16s rDNA 区域序列分析揭示土耳其蜜蜂亚种的多样性。

Diversity of Apis mellifera subspecies from Turkey revealed by sequence analysis of mitochondrial 16s rDNA region.

机构信息

Animal Science Department, Biometry and Genetics Division, Faculty of Agriculture, Selçuk University, 42075 Konya, Turkey.

出版信息

Biochem Genet. 2012 Oct;50(9-10):748-60. doi: 10.1007/s10528-012-9517-1. Epub 2012 May 26.

DOI:10.1007/s10528-012-9517-1

PMID:22639063

Abstract

Mitochondrial DNA sequence variation can be used to infer honeybee evolutionary relationships. In this study, DNA sequence diversity of the mitochondrial 16s rDNA region was investigated in 112 honeybees from 15 populations in Turkey, which is mainly populated with Apis mellifera anatoliaca, A. m. caucasica, and A. m. meda. The study revealed 11 haplotypes for this segment, with 13 variable sites and nine parsimony informative sites. The haplotypes were not discriminated according to their geographical locations in a neighbor-joining dendrogram based on 16s rDNA sequences available in Genbank, but all the haplotypes obtained in this study are clustered with published haplotypes such as A. mellifera TAS (AF214666) and A. m. ligustica (EF116868) and with some unpublished Genbank records (HQ318928, HQ318934, and HQ318938). This study expands the knowledge of the mitochondrial 16s rDNA region, and it presents the first comprehensive sequence analysis of this region in Turkish honeybees.

摘要

线粒体 DNA 序列变异可用于推断蜜蜂的进化关系。在这项研究中，对来自土耳其 15 个种群的 112 只蜜蜂的线粒体 16s rDNA 区域的 DNA 序列多样性进行了研究，这些蜜蜂主要分布在安纳托利亚蜜蜂、高加索蜜蜂和地中海蜜蜂中。该研究揭示了该片段的 11 种单倍型，有 13 个可变位点和 9 个简约信息位点。基于 Genbank 中可用的 16s rDNA 序列构建的邻接树聚类分析并未根据地理位置对单倍型进行区分，但本研究获得的所有单倍型都与已发表的单倍型（如 A. mellifera TAS（AF214666）和 A. m. ligustica（EF116868））以及一些未发表的 Genbank 记录（HQ318928、HQ318934 和 HQ318938）聚类在一起。本研究扩展了对线粒体 16s rDNA 区域的认识，并首次对土耳其蜜蜂该区域进行了全面的序列分析。