Yin L, Ji T
College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Genet Mol Res. 2013 Jun 20;12(2):2002-9. doi: 10.4238/2013.June.20.1.
DNA sequence diversity in the tRNA(leu)-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNA(leu)-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 were new. The mean diversity of haplotypes was 0.752 ± 0.030 (0.378-0.698), and nucleotide diversity was 0.01073 ± 0.00087 (0.00412-0.01123). A neighbor-joining tree was constructed based on 73 sequences of noncoding intergenic regions in the mtDNA of A. cerana from China and other Asian countries; all haplotypes found in China fell into the mainland Asian group. This result does not support the hypothesis that A. cerana indica occurs in southern Yunnan, which was concluded in a recent report based on morphological variation.
对来自中国云南的中华蜜蜂样本的线粒体基因组中tRNA(亮氨酸)-细胞色素氧化酶亚基II（COII）部分的DNA序列多样性进行了研究。对tRNA(亮氨酸)-COII中约480 bp的片段进行了测序，该片段包括一个非编码区和部分COII。非编码区长97 - 98 bp；共发现8种单倍型，其中5种先前已有报道，3种为新发现的。单倍型的平均多样性为0.752±0.030（0.378 - 0.698），核苷酸多样性为0.01073±0.00087（0.00412 - 0.01123）。基于来自中国和其他亚洲国家的中华蜜蜂线粒体DNA中非编码基因间区域的73个序列构建了邻接树；在中国发现的所有单倍型都属于亚洲大陆组。这一结果不支持最近一份基于形态变异得出的关于滇南存在印度蜜蜂的假说。
