Department of Biomolecular Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Biotechnol Lett. 2012 Sep;34(9):1741-4. doi: 10.1007/s10529-012-0960-1. Epub 2012 May 25.
Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADPH. Whole cells of GF12, also catalyzed the hydroxylation of 10 mM 1-adamantanol (1-adOH), to 3.6 mM 1,3,5-ad(OH)(3).
在 24°C 下，用 4 毫升缓冲液（pH 7）洗涤 Kitasatospora sp. GF12 细胞（62mg），催化 60mM1,3-金刚烷二醇[1,3-ad(OH)(2)]的区域选择性羟化，生成 30.9mM1,3,5-金刚烷三醇[1,3,5-ad(OH)(3)]，反应持续 120 小时。向反应混合物中加入 400mM 甘油以循环利用细胞内 NADH/NADPH。GF12 的全细胞也能催化 10mM1-金刚烷醇（1-adOH）的羟化反应，生成 3.6mM1,3,5-ad(OH)(3)。
