用于在形态发生过程中量化集体细胞迁移的工程组织。

Engineered tissues to quantify collective cell migration during morphogenesis.

作者信息

Manivannan Sriram, Gleghorn Jason P, Nelson Celeste M

机构信息

Department of Chemical Engineering, Princeton University, Princeton, NJ, USA.

出版信息

Methods Mol Biol. 2012;886:173-82. doi: 10.1007/978-1-61779-851-1_16.

DOI:10.1007/978-1-61779-851-1_16

PMID:22639261

Abstract

Renal development is a complex process involving the dynamic interplay of over 25 different cell types. One distinct step in this process is the formation of the ureteric tree, which develops from the repeated branching of the ureteric bud. During branching of the ureteric bud, cells migrate collectively in unison to form the complex structure of the tree. Here, we present a microlithography-based 3D culture model in which multiple identical kidney epithelial tissues are used to quantify collective cell migration during the process of branching morphogenesis.

摘要

肾脏发育是一个复杂的过程，涉及超过25种不同细胞类型的动态相互作用。这个过程中的一个独特步骤是输尿管树的形成，它由输尿管芽的反复分支发育而来。在输尿管芽分支过程中，细胞协同集体迁移以形成树状的复杂结构。在这里，我们展示了一种基于微光刻的3D培养模型，其中使用多个相同的肾上皮组织来量化分支形态发生过程中的集体细胞迁移。