Tai Guanping, Hohenstein Peter, Davies Jamie A
University of Edinburgh, Hugh Robson Building, Edinburgh, UK.
Methods Mol Biol. 2012;886:165-71. doi: 10.1007/978-1-61779-851-1_15.
Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the "Immortomouse," which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.
源自胚胎小鼠肾脏的永生化细胞系是探索肾脏发育中的信号通路、形态发生机制和基因功能的有用工具：它们还为有效首轮筛选旨在对抗诸如囊肿形成等肾脏疾病的小分子库提供了一种手段，并且这种基于细胞系的筛选可以显著减少特定研究路线所需的动物数量。本章介绍了一种从“永生小鼠”生成细胞系的简单方法，该小鼠携带一种受干扰素调节启动子控制的温度敏感型SV40抗原。
