Murawski Inga J, Watt Christine L, Gupta Indra R
Department of Human Genetics, Montreal Children's Hospital, McGill University, Montreal, QC, Canada.
Methods Mol Biol. 2012;886:351-62. doi: 10.1007/978-1-61779-851-1_31.
Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) encompass a spectrum of kidney and urinary tract disorders. Here, we describe two assays that can be used to determine if a mouse has vesicoureteric reflux (VUR) or urinary tract obstruction, two urinary tract defects observed in CAKUT. To test for VUR, dye is injected into the mouse bladder and then monitored to determine if it passes retrogradely from the bladder towards the kidneys, indicating the presence of VUR. To test for urinary tract obstruction, the renal pelvis is microinjected with dye and its passage along the urinary tract is monitored to determine if there is evidence of impaired flow along the tract. These methods will facilitate the analysis of CAKUT phenotypes in the mouse.
先天性肾脏和尿路畸形（CAKUT）包括一系列肾脏和尿路疾病。在此，我们描述了两种检测方法，可用于确定小鼠是否患有膀胱输尿管反流（VUR）或尿路梗阻，这是在CAKUT中观察到的两种尿路缺陷。为了检测VUR，将染料注入小鼠膀胱，然后进行监测以确定染料是否从膀胱逆行流向肾脏，这表明存在VUR。为了检测尿路梗阻，将染料微量注射到肾盂，并监测其沿尿路的通过情况，以确定是否有尿路流动受损的证据。这些方法将有助于分析小鼠中的CAKUT表型。
