Laboratoire ‶Glycobiologie et Matrice Extracellulaire Végétale″, UPRES EA 4358, Institut Federatif de Recherche Multidisciplinaire sur les Peptides, Plate-forme de Recherche en Imagerie Cellulaire de Haute Normandie, Université de Rouen Mont Saint Aignan, France.
Front Plant Sci. 2012 Apr 30;3:79. doi: 10.3389/fpls.2012.00079. eCollection 2012.
The Golgi apparatus of eukaryotic cells is known for its central role in the processing, sorting, and transport of proteins to intra- and extra-cellular compartments. In plants, it has the additional task of assembling and exporting the non-cellulosic polysaccharides of the cell wall matrix including pectin and hemicelluloses, which are important for plant development and protection. In this review, we focus on the biosynthesis of complex polysaccharides of the primary cell wall of eudicotyledonous plants. We present and discuss the compartmental organization of the Golgi stacks with regards to complex polysaccharide assembly and secretion using immuno-electron microscopy and specific antibodies recognizing various sugar epitopes. We also discuss the significance of the recently identified Golgi-localized glycosyltransferases responsible for the biosynthesis of xyloglucan (XyG) and pectin.
真核细胞的高尔基体以其在蛋白质的加工、分拣和向细胞内和细胞外隔室运输中的核心作用而闻名。在植物中，它还有组装和输出细胞壁基质中非纤维素多糖的额外任务，包括果胶和半纤维素，这些多糖对于植物的发育和保护很重要。在这篇综述中，我们专注于研究双子叶植物初生细胞壁的复杂多糖的生物合成。我们使用免疫电子显微镜和识别各种糖表位的特异性抗体，展示和讨论高尔基体堆叠在复杂多糖组装和分泌方面的区室组织。我们还讨论了最近发现的负责木葡聚糖 (XyG) 和果胶生物合成的定位于高尔基体的糖基转移酶的重要性。
