Institute of Basic Theory, China Academy of Chinese Medical Sciences, South Small Street 16, Dongzhimennei, Dongcheng District, Beijing 100700, Peoples Republic of ChineChina.
BMC Complement Altern Med. 2012 May 28;12:67. doi: 10.1186/1472-6882-12-67.
The objective of this study was to evaluate the effects of herbal medicines, such as Radix Dipsaci (RDD), Pyrola Herb (PHD), and Cynomorium songaricum decoction (CSD), on osteoporotic rats induced by ovariectomy (OVX).
OVX or sham operations were performed on 69 virgin Wistar rats that were divided into six groups: sham (sham, n = 12), OVX control group (OVX, n = 12), and OVX rats with treatments (diethylstilbestrol, E2, n = 12; RDD, n = 11, PHD, n = 11, and CSD, n = 11). Non-surgical rats served as normal control (NC, n = 12). The treatments began four weeks after surgery and lasted for 12 weeks. Bone mass and bone turnover were analyzed by histomorphometry. Levels of protein expression and mRNA of OPG and RANKL in osteoblasts (OB) and bone marrow stromal cells (bMSC) were evaluated by immunohistochemistry and in situ hybridization.
Compared to NC and sham rats, trabecular bone formation was significantly reduced in OVX rats, but restored in E2-treated rats. Treatment with either RDD or PHD enhanced trabecular bone formation remarkably. No significant change of bone formation was observed in CSD-treated rats. OPG expression of protein and mRNA was reduced significantly in OB and bMSC of OVX control rats. RANKL expression of protein and mRNA was increased significantly in OB and bMSC of OVX control rats. These effects were substantially reversed (increased in OPG and decreased in RANKL) by treatment with E2, RDD, or PHD in OB and bMSC of OVX rats. No significant changes in either OPG or RANKL expression were observed in OB and bMSC of OVX rats treated with CSD.
Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC. This suggests that RDD and PHD may be used as alternative therapeutic agents for postmenopausal osteoporosis.
本研究旨在评估中药如独活（RDD）、鹿衔草（PHD）和肉苁蓉汤（CSD）对卵巢切除（OVX）诱导的骨质疏松症大鼠的影响。
对 69 只处女 Wistar 大鼠进行 OVX 或假手术，将其分为 6 组：假手术（sham，n=12）、OVX 对照组（OVX，n=12）、OVX 治疗组（己烯雌酚，E2，n=12；RDD，n=11，PHD，n=11，CSD，n=11）。未手术的大鼠作为正常对照组（NC，n=12）。手术后 4 周开始治疗，持续 12 周。通过组织形态计量学分析骨量和骨转换。通过免疫组化和原位杂交评估成骨细胞（OB）和骨髓基质细胞（bMSC）中 OPG 和 RANKL 的蛋白和 mRNA 水平。
与 NC 和 sham 大鼠相比，OVX 大鼠的小梁骨形成明显减少，但 E2 治疗组得到恢复。RDD 或 PHD 治疗可显著增强小梁骨形成。CSD 治疗组的骨形成无明显变化。OVX 对照组大鼠 OB 和 bMSC 中 OPG 的蛋白和 mRNA 表达明显减少。OVX 对照组大鼠 OB 和 bMSC 中 RANKL 的蛋白和 mRNA 表达明显增加。这些作用在 OB 和 bMSC 的 OVX 大鼠中通过 E2、RDD 或 PHD 的治疗得到了显著逆转（OPG 增加，RANKL 减少）。CSD 治疗的 OVX 大鼠 OB 和 bMSC 中 OPG 或 RANKL 的表达均无明显变化。
本研究表明，RDD 和 PHD 通过刺激 OB 和 bMSC 中 OPG 的过度表达和 RANKL 的下调来增加骨形成。这表明 RDD 和 PHD 可能可作为绝经后骨质疏松症的替代治疗药物。
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