Department of Pharmacology, Chonbuk National University, Jeonju, Chonbuk, South Korea.
BMC Complement Altern Med. 2011 Nov 28;11:120. doi: 10.1186/1472-6882-11-120.
Salvia miltiorrhiza (SM) has long been used as a traditional oriental medicine for cardiovascular disease. Accumulating evidence also indicates that SM has anti-osteoporotic effects. This study was conducted to examine the SM-induced anti-osteoporotic effect and its possible mechanisms with various doses of SM.
We studied Sprague-Dawley female rats aged 12 weeks, divided into six groups: sham-operated control (SHAM), OVX rats supplemented with SM (1, 3, 10 and 30 mg/kg) orally for 8 weeks. At the end of the experiment, blood samples were collected and biochemistry analysis was performed. Specimens from both tibia and liver were processed for light microscopic examination. DEXA and μ-CT analyses of the tibia were also performed.
SM treatment significantly ameliorated the decrease in BMD and trabecular bone mass according to DEXA and trabecular bone architecture analysis of trabecular bone structural parameters by μ-CT scanning. In serum biochemical analysis, SM decreased the released TRAP-5b, an osteoclast activation marker and oxidative stress parameters including MDA and NO induced by OVX.
The preventive effect of SM was presumably due to its anti-oxidative stress partly via modulation of osteoclast maturation and number. In current study, SM appears to be a promising osteoporosis therapeutic natural product.
丹参（SM）长期以来一直被用作治疗心血管疾病的传统东方药物。越来越多的证据表明，SM 具有抗骨质疏松作用。本研究旨在研究不同剂量 SM 对骨质疏松的预防作用及其可能的机制。
我们研究了 12 周龄的 Sprague-Dawley 雌性大鼠，将其分为六组：假手术对照组（SHAM）、OVX 大鼠口服 SM（1、3、10 和 30mg/kg）8 周。实验结束时，采集血样进行生化分析。对胫骨和肝脏标本进行光镜检查。还对胫骨进行了 DEXA 和 μ-CT 分析。
SM 治疗可显著改善 DEXA 分析和 μ-CT 扫描的小梁骨结构参数分析中 BMD 和小梁骨量的减少。在血清生化分析中，SM 降低了 OVX 诱导的破骨细胞激活标志物 TRAP-5b 和氧化应激参数 MDA 和 NO 的释放。
SM 的预防作用可能部分归因于其抗氧化应激作用，部分归因于对破骨细胞成熟和数量的调节。在本研究中，SM 似乎是一种有前途的骨质疏松症治疗天然产物。
