Interdisciplinary Nanoscience Center, Faculty of Science, Aarhus University, Ny Munkegade, 8000 Aarhus C, Denmark.
Langmuir. 2012 Jun 12;28(23):8594-9. doi: 10.1021/la301441t. Epub 2012 May 31.
The development of cost-effective methodologies for the precise nanometer-scale positioning of biomolecules permits the low-cost production of various biofunctional devices for a range of biomedical and nanotechnological applications. By combining colloidal lithography and the mussel-inspired multifunctional polydopamine coating, we present a novel parallel benchtop method that allows rapid nanoscale patterning of proteins without the need for electrically powered equipment in the fabrication process. The PDA-immobilized binary nanopattern consisting of BSA surrounded by PLL-g-PEG is fabricated over a large area, and the integrity of the pattern is confirmed using AFM and FM.
开发经济高效的方法来实现生物分子的精确纳米级定位，使得各种生物功能设备能够以低成本生产，从而应用于一系列生物医学和纳米技术领域。本研究通过将胶体光刻技术和受贻贝启发的多功能聚多巴胺涂层相结合，提出了一种新颖的平行台式方法，该方法可在无需在制造过程中使用电动设备的情况下，快速对蛋白质进行纳米级图案化处理。通过 AFM 和 FM 确认了由 PLL-g-PEG 包围的 BSA 组成的 PDA 固定的二元纳米图案在大面积上的完整性。
