Latvian Institute of Organic Synthesis, Laboratory of Pharmaceutical Pharmacology, Aizkraukles str 21, Riga, Latvia.
J Neurosci Methods. 2012 Jul 15;208(2):197-204. doi: 10.1016/j.jneumeth.2012.05.018. Epub 2012 May 26.
Middle cerebral artery occlusion (MCAO) is the most commonly used method to study the neurological and histological outcomes and the pathological mechanisms of ischaemic stroke. The current work compares sensorimotor and cognitive deficits and the infarct volume in rats following a transient 90- or 120-min MCAO, which allows the appropriate behavioural tests to be chosen based on the goal and design of the experiment. In the beam-walking test, we found significant differences between the 90- and 120-min MCAO groups in the number of foot faults made with the impaired hindlimb on post-stroke days 3, 7 and 14. In the cylinder test, a difference between the 90- and 120-min groups was observed on post-operation day 14. The responses to tactile and proprioceptive stimulation were impaired to a similar extent after 90- and 120-min MCAO in the vibrissae-evoked forelimb-placing and limb-placing tests. Moreover, we found significant memory impairment in the 120-min MCAO group 6 days after the acquisition trial. The brain tissue damage was significantly higher after 120-min occlusion of the MCA compared with 90-min occlusion; the infarct volumes were 13% and 25% of the contralateral hemispheres, respectively. In conclusion, both the 90- and 120-min occlusion models result in a significant impairment of sensorimotor, tactile and proprioceptive function, but memory impairment is only observed in the 120-min MCAO group. The beam-walking and cylinder tests detected neurological dysfunction after the 120-min MCAO, whereas the limb-placing and vibrissae-evoked forelimb-placing tests were able to evaluate the neurological dysfunction in rats after 90- and 120-min MCAO.
大脑中动脉闭塞（MCAO）是研究缺血性中风的神经和组织学结果及病理机制最常用的方法。目前的工作比较了短暂 90 分钟或 120 分钟 MCAO 后大鼠的感觉运动和认知缺陷以及梗死体积，这使得可以根据实验的目的和设计选择适当的行为测试。在走棒试验中，我们发现，在脑缺血后第 3、7 和 14 天，受损后肢的足误次数在 90 分钟和 120 分钟 MCAO 组之间存在显著差异。在圆筒试验中，在手术后第 14 天观察到 90 分钟和 120 分钟组之间的差异。在触须诱发的前肢放置和肢体放置试验中，90 分钟和 120 分钟 MCAO 后，对触觉和本体感觉刺激的反应受损程度相似。此外，我们发现，在获得试验后 6 天，120 分钟 MCAO 组的记忆损伤明显。与 90 分钟 MCAO 相比，MCA 120 分钟闭塞后的脑组织损伤明显更高；梗死体积分别为对侧半球的 13%和 25%。总之，90 分钟和 120 分钟的闭塞模型均导致感觉运动、触觉和本体感觉功能明显受损，但只有在 120 分钟 MCAO 组中观察到记忆损伤。在 120 分钟 MCAO 后，走棒试验和圆筒试验检测到神经功能障碍，而肢体放置试验和触须诱发的前肢放置试验能够评估 90 分钟和 120 分钟 MCAO 后大鼠的神经功能障碍。
