Zhao Lin, Feng Zhihui, Hu Bin, Chi Xiaoyun, Jiao Shuxian
Institute for Transfusion Medicine, Qingdao Blood Center, Qingdao, China.
Hepatogastroenterology. 2012 Nov-Dec;59(120):2389-94. doi: 10.5754/hge12288.
BACKGROUND/AIMS: To investigate the feasibility and mechanism of liver damage repair using BMSCs, we investigated the potential for BMSCs in recovery from liver damage, using the CCl4-induced rat model for liver damage.
Phenotypings of BMSCs of the third generation were analyzed by flow cytometry. BMSCs labelled by BrdU were infused via the tail vein of CCl4-induced rat model. The labeling yields ob-served were detected by flow cytometry and liver samples were taken for immunohistochemistry. Concentration changes of AST, ALT and AKP were observed by a serum enzymology test after transplantation.
The BrdU' cells in recipient livers were detect-ed on day 7 after BMSCs transplantation and engraft-ed cells were found in the peri-portal regions of the hepatic lobule and on day 14 more of them spread throughout the lobules. BMSCs engraftments were detected on whole hepatic parenchyma. The serum lev-els of ALT, AST and AKP in group 3 were all lower than those of group 2 from the 7h to 28th day.
Ex vivo-expanded BMSCs were capable of relocating to the chemically-injured liver. Transplantation of these pluripotent stem cells appeared to improve serum indices of liver function.
背景/目的：为了研究使用骨髓间充质干细胞（BMSCs）修复肝损伤的可行性及机制，我们采用四氯化碳诱导的大鼠肝损伤模型，研究了BMSCs在肝损伤恢复中的潜力。
通过流式细胞术分析第三代BMSCs的表型。将用5-溴脱氧尿嘧啶核苷（BrdU）标记的BMSCs经尾静脉注入四氯化碳诱导的大鼠模型。通过流式细胞术检测观察到的标记率，并取肝脏样本进行免疫组织化学检测。移植后通过血清酶学检测观察天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（AKP）的浓度变化。
BMSCs移植后第7天在受体肝脏中检测到BrdU+细胞，在肝小叶的门静脉周围区域发现了植入的细胞，第14天时更多的细胞扩散到整个小叶。在整个肝实质中均检测到BMSCs植入。从第7小时至第28天，第3组的ALT、AST和AKP血清水平均低于第2组。
体外扩增的BMSCs能够迁移至化学损伤的肝脏。这些多能干细胞的移植似乎改善了肝功能的血清指标。
