Serpa Jason J, Parker Carol E, Petrotchenko Eveniy V, Han Jun, Pan Jingxi, Borchers Christoph H
University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, Victoria, BC V8Z 7X8, Canada.
Eur J Mass Spectrom (Chichester). 2012;18(2):251-67. doi: 10.1255/ejms.1178.
Structural proteomics is the application of protein chemistry and modern mass spectrometric techniques to problems such as the characterization of protein structures and assemblies and the detailed determination of protein-protein interactions. The techniques used in structural proteomics include crosslinking, photoaffinity labeling, limited proteolysis, chemical protein modification and hydrogen/deuterium exchange, all followed by mass spectrometric analysis. None of these methods alone can provide complete structural information, but a "combination" of these complementary approaches can be used to provide enough information for answering important biological questions. Structural proteomics can help to determine, for example, the detailed structure of the interfaces between proteins that may be important drug targets and the interactions between proteins and ligands. In this review, we have tried to provide a brief overview of structural proteomics methodologies, illustrated with examples from our laboratory and from the literature.
结构蛋白质组学是将蛋白质化学和现代质谱技术应用于诸如蛋白质结构和组装体的表征以及蛋白质-蛋白质相互作用的详细测定等问题。结构蛋白质组学中使用的技术包括交联、光亲和标记、有限蛋白酶解、化学蛋白质修饰和氢/氘交换，所有这些之后都进行质谱分析。这些方法单独一种都不能提供完整的结构信息，但这些互补方法的“组合”可用于提供足够的信息来回答重要的生物学问题。例如，结构蛋白质组学有助于确定可能是重要药物靶点的蛋白质之间界面的详细结构以及蛋白质与配体之间的相互作用。在本综述中，我们试图简要概述结构蛋白质组学方法，并结合我们实验室和文献中的实例进行说明。
