基于现代质谱的结构蛋白质组学。

Modern mass spectrometry-based structural proteomics.

机构信息

University of Victoria-Genome British Columbia Proteomics Centre, Victoria, British Columbia, Canada.

University of Victoria-Genome British Columbia Proteomics Centre, Victoria, British Columbia, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, Victoria, British Columbia, Canada.

出版信息

Adv Protein Chem Struct Biol. 2014;95:193-213. doi: 10.1016/B978-0-12-800453-1.00006-3.

DOI:10.1016/B978-0-12-800453-1.00006-3

PMID:24985773

Abstract

Recent developments in the modern mass spectrometry of proteins and peptides have resulted in significant progress in structural proteomics techniques for studying protein structure. A variety of protein structural questions, ranging from defining protein interaction networks to the study of conformational changes and the structure of single proteins, can be addressed using multiple mass spectrometry-based structural proteomics approaches. Each technique provides specific structural information which can be used as experimental structural constraints in protein structure modeling. Here, we describe recent developments in limited proteolysis, surface modification, hydrogen-deuterium exchange, ion mobility, and cross-linking-all combined with modern mass spectrometric techniques-for the studying protein structure.

摘要

近年来，蛋白质和肽的现代质谱技术取得了显著进展，为研究蛋白质结构的结构蛋白质组学技术带来了重大进步。多种蛋白质结构问题，从定义蛋白质相互作用网络到构象变化和单个蛋白质结构的研究，都可以使用多种基于质谱的结构蛋白质组学方法来解决。每种技术都提供了特定的结构信息，可以用作蛋白质结构建模的实验结构约束。在这里，我们描述了有限蛋白水解、表面修饰、氢氘交换、离子淌度和交联的最新进展，所有这些都与现代质谱技术相结合，用于研究蛋白质结构。