Veziris Nicolas
UPMC université Paris-6, ER5, EA 1541, Laboratoire de bactériologie-hygiéne, F-75005 Paris.
Rev Prat. 2012 Apr;62(4):490-2.
Today, microscopic examination and culture are still essential for active tuberculosis diagnosis and therapeutic decisions. Nucleic acid amplification and line probe assays speed up identification and susceptibility testing of mycobacteria in AFB smear positive specimens or in culture. They are also efficient for comparison of M. tuberculosis strains with each other (genotyping). On the other hand, today, molecular tests are not relevant for the diagnosis in smear negative specimens and even more for diagnosis of culture-negative tuberculosis.
如今，显微镜检查和培养对于活动性结核病的诊断及治疗决策仍然至关重要。核酸扩增和线性探针检测可加快对抗酸杆菌涂片阳性标本或培养物中分枝杆菌的鉴定和药敏试验。它们对于结核分枝杆菌菌株之间的比较（基因分型）也很有效。另一方面，如今分子检测对于涂片阴性标本的诊断并不适用，对于培养阴性结核病的诊断更是如此。
