Sorokina E Iu, Chernyshova O N
Vopr Pitan. 2012;81(1):44-8.
A quantitative real-time multiplex polymerase chain reaction method was applied to the detection and quantification of MON863 and MON810 in stacked genetically modified maize MON 810xMON 863. The limit of detection was approximately 0,1%. The accuracy of the quantification, measured as bias from the accepted value and the relative repeatability standard deviation, which measures the intra-laboratory variability, were within 25% at each GM-level. A method verification has demonstrated that the MON 863 and the MON810 methods can be equally applied in quantification of the respective events in stacked MON810xMON 863.
采用定量实时多重聚合酶链反应方法对转基因玉米MON 810xMON 863中MON863和MON810进行检测和定量分析。检测限约为0.1%。以与公认值的偏差衡量的定量准确度以及衡量实验室内变异性的相对重复性标准偏差在每个转基因水平下均在25%以内。方法验证表明，MON 863和MON810方法可同等用于定量分析叠加型MON810xMON 863中各自的事件。
