Dew R K, Boissonneault G A, Gay N, Boling J A, Cross R J, Cohen D A
Department of Animal Sciences, College of Agriculture, University of Kentucky, Lexington 40546.
Vet Immunol Immunopathol. 1990 Nov;26(3):285-95. doi: 10.1016/0165-2427(90)90097-c.
Experiments were conducted with rats and mice to evaluate the effect of the consumption of endophyte (Acremonium coenophialum) and associated toxin(s) infected tall fescue on humoral and cellular aspects of immune function. Treatment diets were: (1) rodent chow (RC) or (2) rodent chow mixed 1:1 (w/w) with endophyte infected (E+) or (3) non-infected (E-) tall fescue seed. Rats fed the E+ diet in experiment 1 (43 days) exhibited a lower (P less than 0.05) serum titer to sheep red blood cell (SRBC) immunization than those fed the E- diet (38.4 vs 131.3). The E+ rats also had lower (P less than 0.01) white cell counts than either RC or E- groups (5225 vs 8959 and 7491/mm3). Spleen cells from mice fed the E+ diet for 37 days exhibited a reduced (P less than 0.05) response to the mitogens Concanavalin A and lipopolysaccharide. Flow cytometric analysis revealed a significant (P less than 0.01) 42% increase in T suppressor cell numbers in spleens of mice fed the E+ vs RC diets.
用大鼠和小鼠进行实验，以评估食用内生真菌（内生镰刀菌）和相关毒素感染的高羊茅对免疫功能的体液和细胞方面的影响。处理日粮如下：（1）啮齿动物饲料（RC）或（2）与内生真菌感染（E+）或（3）未感染（E-）的高羊茅种子按1:1（w/w）混合的啮齿动物饲料。在实验1（43天）中，喂食E+日粮的大鼠对绵羊红细胞（SRBC）免疫的血清效价低于喂食E-日粮的大鼠（P<0.05）（38.4对131.3）。E+组大鼠的白细胞计数也低于RC组或E-组（P<0.01）（5225对8959和7491/mm³）。喂食E+日粮37天的小鼠的脾细胞对促细胞分裂剂刀豆球蛋白A和脂多糖的反应降低（P<0.05）。流式细胞仪分析显示，喂食E+日粮的小鼠脾脏中T抑制细胞数量比喂食RC日粮的小鼠显著增加（P<0.01）42%。
