Department of Obstetrics and Gynecology, Keelung Chang Gung Memorial Hospital, Keelung and Chang Gung University, Taiwan.
Climacteric. 2013 Feb;16(1):154-60. doi: 10.3109/13697137.2012.672496. Epub 2012 May 29.
To determine whether estrogen regulates mesenchymal stem cell (MSC) activity in bone marrow from osteoporotic postmenopausal women.
MSCs were collected from bone marrows which were aspirated simultaneously during iliac bone graft procedures in spine fusion surgery in osteoporotic postmenopausal women. We investigated proliferation, differentiation, osteogenic activity, and estrogen receptor (ER) α and β mRNA expression of primary culture MSCs isolated from four osteoporotic postmenopausal women, treated in vitro with or without 17β-estradiol. The expression of alkaline phosphatase (ALP), osteocalcin, interleukin-6, ERα and ERβ mRNA was evaluated.
The expression of ALP and osteocalcin mRNA was detected during the cultures of MSCs and was observed to increase up to day 20. As compared with MSCs not treated with estradiol, a significant increase in DNA content, ERα mRNA, and ALP mRNA expression was observed in cultures with estradiol. The mRNA expression of osteocalcin and interleukin-6 was significantly lower in MSCs treated with estradiol than those without estradiol. There was no significant difference in the mRNA expression of ERβ between MSCs cultured with and without estradiol.
In the proper environment, MSCs from osteoporotic women can differentiate into osteoblasts and estrogen enhances the osteogenic activity possibly via ERα activity.
确定雌激素是否调节骨质疏松绝经后女性骨髓间充质干细胞（MSC）的活性。
从骨质疏松绝经后女性脊柱融合手术中髂骨取骨过程中同时抽吸的骨髓中收集 MSC。我们研究了从 4 名骨质疏松绝经后女性分离的原代培养 MSC 的增殖、分化、成骨活性以及雌激素受体（ER）α和β mRNA 表达，体外用或不用 17β-雌二醇处理。评估碱性磷酸酶（ALP）、骨钙素、白细胞介素 6、ERα 和 ERβ mRNA 的表达。
在 MSC 的培养过程中检测到 ALP 和骨钙素 mRNA 的表达，并观察到其在第 20 天之前增加。与未用雌二醇处理的 MSC 相比，用雌二醇处理的 MSC 中 DNA 含量、ERα mRNA 和 ALP mRNA 表达显著增加。用雌二醇处理的 MSC 中骨钙素和白细胞介素 6 的 mRNA 表达明显低于未用雌二醇处理的 MSC。用雌二醇和未用雌二醇培养的 MSC 之间 ERβ mRNA 的表达没有显著差异。
在适当的环境下，骨质疏松女性的 MSC 可以分化为成骨细胞，雌激素可能通过 ERα 活性增强成骨活性。
