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雌激素抑制微小RNA-30b促进骨髓间充质干细胞成骨分化

Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells.

作者信息

Liu Guanqi, Lu Yeming, Mai Zhihui, Liu Runheng, Peng Zhuli, Chen Lin, Chen Zheng, Wang Ruizhi, Ai Hong

机构信息

Department of Stomatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.

出版信息

Stem Cells Int. 2019 Apr 4;2019:7547506. doi: 10.1155/2019/7547506. eCollection 2019.

DOI:10.1155/2019/7547506
PMID:31089333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6476012/
Abstract

MicroRNAs (miRNAs) have been widely demonstrated to interact with multiple cellular signaling pathways and to participate in a wide range of physiological processes. Estradiol-17 (E2) is the most potent and prevalent endogenous estrogen that plays a vital role in promoting bone formation and reducing bone resorption. Currently, little is known about the regulation of miRNAs in E2-induced osteogenic differentiation. In the present study, the primary bone marrow mesenchymal stem cells from rats (rBMSCs) were isolated and incubated with E2, followed by miRNA profiling. The microarray showed that 29 miRNAs were differentially expressed in response to E2 stimulation. Further verification by real-time reverse-transcriptase polymerase chain reaction revealed that E2 enhanced the expression of let-7b and miR-25 but suppressed the miR-30b expression. Moreover, a gain-of-function experiment confirmed that miR-30b negatively regulated the E2-induced osteogenic differentiation. These data suggest an important role of miRNAs in osteogenic differentiation.

摘要

微小RNA（miRNA）已被广泛证明可与多种细胞信号通路相互作用，并参与广泛的生理过程。雌二醇-17（E2）是最有效且普遍存在的内源性雌激素，在促进骨形成和减少骨吸收方面发挥着至关重要的作用。目前，关于miRNA在E2诱导的成骨分化中的调控作用知之甚少。在本研究中，分离大鼠原代骨髓间充质干细胞（rBMSC）并用E2孵育，随后进行miRNA谱分析。微阵列显示，有29种miRNA在E2刺激下差异表达。通过实时逆转录聚合酶链反应进一步验证发现，E2增强了let-7b和miR-25的表达，但抑制了miR-30b的表达。此外，功能获得实验证实miR-30b负向调节E2诱导的成骨分化。这些数据表明miRNA在成骨分化中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/397b7ca33017/SCI2019-7547506.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/a4724b1548d4/SCI2019-7547506.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/b105affef385/SCI2019-7547506.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/397b7ca33017/SCI2019-7547506.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/a4724b1548d4/SCI2019-7547506.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/49abbdee6617/SCI2019-7547506.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/884894d7b723/SCI2019-7547506.003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3745/6476012/397b7ca33017/SCI2019-7547506.008.jpg

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本文引用的文献

1
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J Cell Physiol. 2018 Aug;233(8):5574-5588. doi: 10.1002/jcp.26514. Epub 2018 Mar 9.
2
AntimiR-30b Inhibits TNF-α Mediated Apoptosis and Attenuated Cartilage Degradation through Enhancing Autophagy.抗微小RNA-30b通过增强自噬抑制肿瘤坏死因子-α介导的细胞凋亡并减轻软骨降解。
Cell Physiol Biochem. 2016;40(5):883-894. doi: 10.1159/000453147. Epub 2016 Dec 7.
3
MicroRNAs in regulation of osteogenic differentiation of mesenchymal stem cells.
β-淀粉样肽治疗通过Wnt信号通路逆转下颌髁突骨质流失。
Bone Rep. 2024 Jul 8;22:101788. doi: 10.1016/j.bonr.2024.101788. eCollection 2024 Sep.
4
MiR-30 Family: A Novel Avenue for Treating Bone and Joint Diseases?miR-30 家族：治疗骨与关节疾病的新途径？
Int J Med Sci. 2023 Feb 13;20(4):493-504. doi: 10.7150/ijms.81990. eCollection 2023.
5
LncRNA HOTTIP impacts the proliferation and differentiation of fibroblast-like synoviocytes in ankylosing spondylitis through the microRNA-30b-3p/PGK1 axis.长链非编码 RNA HOTTIP 通过 microRNA-30b-3p/PGK1 轴影响强直性脊柱炎成纤维样滑膜细胞的增殖和分化。
J Orthop Surg Res. 2023 Mar 24;18(1):237. doi: 10.1186/s13018-023-03653-4.
6
Recent advances on small molecules in osteogenic differentiation of stem cells and the underlying signaling pathways.近年来小分子在干细胞成骨分化及相关信号通路中的作用研究进展。
Stem Cell Res Ther. 2022 Nov 12;13(1):518. doi: 10.1186/s13287-022-03204-4.
7
Knockdown of FOXA1 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the ERK1/2 signalling pathway.敲低 FOXA1 部分通过激活 ERK1/2 信号通路增强人骨髓间充质干细胞的成骨分化。
Stem Cell Res Ther. 2022 Sep 5;13(1):456. doi: 10.1186/s13287-022-03133-2.
8
Dopamine Suppresses Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells via AKT/GSK-3/-Catenin Signaling Pathway.多巴胺通过AKT/GSK-3/β-连环蛋白信号通路抑制大鼠骨髓间充质干细胞的成骨分化。
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9
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Estrogen Signaling Dictates Musculoskeletal Stem Cell Behavior: Sex Differences in Tissue Repair.雌激素信号决定了肌肉骨骼干细胞的行为：组织修复中的性别差异。
Tissue Eng Part B Rev. 2022 Aug;28(4):789-812. doi: 10.1089/ten.TEB.2021.0094. Epub 2022 Jan 6.
微小RNA对间充质干细胞成骨分化的调控
Cell Tissue Res. 2017 May;368(2):229-238. doi: 10.1007/s00441-016-2462-2. Epub 2016 Jul 18.
4
17β-estradiol differently affects osteogenic differentiation of mesenchymal stem/stromal cells from adipose tissue and bone marrow.17β-雌二醇对来自脂肪组织和骨髓的间充质干/基质细胞的成骨分化有不同影响。
Differentiation. 2016 Dec;92(5):291-297. doi: 10.1016/j.diff.2016.04.001. Epub 2016 Apr 14.
5
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J Cell Physiol. 2017 Jan;232(1):182-91. doi: 10.1002/jcp.25406. Epub 2016 Apr 26.
6
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Drug Dev Res. 2015 Aug;76(5):235-45. doi: 10.1002/ddr.21260. Epub 2015 Jul 27.
7
Calcium ion-induced formation of β-sheet/-turn structure leading to alteration of osteogenic activity of bone morphogenetic protein-2.钙离子诱导β-折叠/β-转角结构的形成，导致骨形态发生蛋白-2的成骨活性改变。
Sci Rep. 2015 Jul 27;5:12694. doi: 10.1038/srep12694.
8
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Exp Cell Res. 2015 Jul 1;335(1):107-14. doi: 10.1016/j.yexcr.2015.04.020. Epub 2015 May 7.
9
mir-21 overexpressing mesenchymal stem cells accelerate fracture healing in a rat closed femur fracture model.过表达mir-21的间充质干细胞在大鼠闭合性股骨骨折模型中加速骨折愈合。
Biomed Res Int. 2015;2015:412327. doi: 10.1155/2015/412327. Epub 2015 Mar 23.
10
Estrogen Modulates Bone Morphogenetic Protein-Induced Sclerostin Expression Through the Wnt Signaling Pathway.雌激素通过Wnt信号通路调节骨形态发生蛋白诱导的硬化蛋白表达。
Tissue Eng Part A. 2015 Jul;21(13-14):2076-88. doi: 10.1089/ten.TEA.2014.0585. Epub 2015 May 26.