CD133(+) 球体形成亚群的 OVCAR3 人卵巢癌细胞系的 microRNA 谱分析。

MicroRNA profiling of a CD133(+) spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line.

机构信息

Institute of Women's Life Medical Science, Women's Cancer Clinic, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, South Korea.

出版信息

BMC Med Genomics. 2012 May 29;5:18. doi: 10.1186/1755-8794-5-18.

BACKGROUND

Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133(+) and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133(+) spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line.

METHODS

Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133(+) OVCAR3 cells with > 98% purity via cell sorting, miRNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile.

RESULTS

We found 37 differentially expressed miRNAs in the CD133(+) spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181.

CONCLUSIONS

Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.

背景

癌症干细胞（CSCs）被认为是肿瘤复发的根源，因为它们具有干细胞样特性。微小 RNA（miRNA）调节正常干细胞和 CSCs，miRNA 的失调在肿瘤发生中起着重要作用。CD133(+)和球体形成已被报道为卵巢 CSCs 的主要特征之一。因此，我们确定了 OVCAR3 人卵巢癌细胞系 CD133(+)球体形成亚群的 miRNA 表达谱。