Witt C, Winsel K, Apostoloff E, Brenke A, Hiepe F, Renner H
Klinik für Innere Medizin Theodor Brugsch, Humboldt-Universität Berlin, DDR.
Z Erkr Atmungsorgane. 1990;175(2):81-7.
The purpose of the study was to determine the role of reactive oxygen metabolites in the pathogenesis of interstitial lung involvement in 13 patients with collagen vascular diseases (CVD) in comparison with 10 control subjects. The cellular activity of bronchoalveolar lavage (BAL) cells was measured by means of chemiluminescence (CL), spontaneously and after challenge of the cells with opsonized zymosan (simultaneously luminol- and lucigenin-dependent). The findings show an increase of the spontaneous and stimulated CL of BAL cells in CVD, more pronounced in luminol-dependent CL. The luminol-dependent CL correlates with neutrophils in BAL cell differentiation. The results suggest that reactive oxygen metabolites take part in interstitial lung involvement in patients with CVD. Therefore the measurement CL of BAL cells is useful in addition to BAL cell differentiation in diagnostic of interstitial lung involvement in CVD.
本研究的目的是确定13例胶原血管病（CVD）患者与10名对照受试者相比，活性氧代谢产物在间质性肺受累发病机制中的作用。通过化学发光（CL）测量支气管肺泡灌洗（BAL）细胞的细胞活性，分别测量自发的以及用调理酵母聚糖刺激细胞后（同时依赖鲁米诺和光泽精）的细胞活性。研究结果显示，CVD患者BAL细胞的自发和刺激CL均增加，在依赖鲁米诺的CL中更为明显。依赖鲁米诺的CL与BAL细胞分化中的中性粒细胞相关。结果表明，活性氧代谢产物参与了CVD患者的间质性肺受累。因此，除了BAL细胞分化外，测量BAL细胞的CL对于诊断CVD患者的间质性肺受累也很有用。
