Ward C, Kelly C A, Stenton S C, Duddridge M, Hendrick D J, Walters E H
Chest Unit, Newcastle General Hospital, University of Newcastle upon Tyne, England.
Eur Respir J. 1990 Oct;3(9):1008-14.
The relationship between differential cell counts and latex-stimulated luminol and lucigenin-amplified chemiluminescence (CL) was investigated by mixing alveolar macrophages (AM) obtained at bronchoalveolar lavage (BAL) with allogeneic peripheral blood neutrophils (PMN) in varying proportions. In 5 non-asthmatic subjects, the mean luminol-amplified CL increased linearly from 2.1 (0.9 SEM) x 10(5) counts per second (cps) with less than 2% PMN, greater than 96% AM to 47.3 (11.1 SEM) x 10(5) cps with greater than 94% PMN, 0% AM (r = 0.996, p less than 0.001). The regression had a y-intercept indistinguishable from 0 cps, suggesting that luminol-amplified CL exclusively reflected PMN activity. Using the same technique, the mean lucigenin-amplified CL showed a fall from 35 (2.3 SEM) x 10(5) cps with a cell population of greater than 96% AM, less than 2% PMN to 20 (2.3 SEM) x 10(5) cps with 0% AM, greater than 94% PMN. Both PMN and AM appeared to contribute to lucigenin-amplified CL, with AM contributing approximately 1.7 times as much activity per cell as PMN. Lucigenin-amplified CL appeared to be an appropriate technique for measuring AM activity when the proportion of PMN in mixed cell populations was small. A linear relationship was found between percent PMN count and luminol-amplified CL measured in a mixed BAL cell population from asthmatic subjects (p less than 0.01) and non-asthmatic controls (p less than 0.01). The slope of this regression line was significantly greater for subjects with asthma than for control subjects (p less than 0.01), suggesting a uniform increase in PMN activity in cells obtained from asthmatic airways.
通过将支气管肺泡灌洗（BAL）获取的肺泡巨噬细胞（AM）与同种异体外周血中性粒细胞（PMN）按不同比例混合，研究了细胞分类计数与乳胶刺激的鲁米诺和光泽精增强化学发光（CL）之间的关系。在5名非哮喘受试者中，鲁米诺增强的CL平均值从PMN低于2%、AM高于96%时的2.1（0.9标准误）×10⁵每秒计数（cps）呈线性增加，到PMN高于94%、AM为0%时的47.3（11.1标准误）×10⁵ cps（r = 0.996，p小于0.001）。该回归线的y轴截距与0 cps无明显差异，表明鲁米诺增强的CL仅反映PMN活性。使用相同技术，光泽精增强的CL平均值从细胞群体中AM高于96%、PMN低于2%时的35（2.3标准误）×10⁵ cps下降到AM为0%、PMN高于94%时的20（2.3标准误）×10⁵ cps。PMN和AM似乎都对光泽精增强的CL有贡献，AM每个细胞的活性贡献约为PMN的1.7倍。当混合细胞群体中PMN比例较小时，光泽精增强的CL似乎是测量AM活性的合适技术。在哮喘受试者（p小于0.01）和非哮喘对照（p小于0.01）的混合BAL细胞群体中，PMN计数百分比与测量的鲁米诺增强的CL之间发现了线性关系。哮喘受试者的这条回归线斜率显著大于对照受试者（p小于0.01），表明从哮喘气道获取的细胞中PMN活性均匀增加。
