Almazov V A, Afanas'ev B V, Shatrov V A, Zubarovskaia L S, Efimov K V, Zaritskiĭ A Iu, Simbirtseva N Iu
Vestn Akad Med Nauk SSSR. 1990(9):37-41.
The influence of human fetal bone (FB) on the hemopoietic and stromal cells were studied in the morphometric assay of the bone marrow trephine biopsy samples after a preliminary cultivation using the Marbrook system for 1-5 weeks. The rat FB effect on the hemopoiesis and survival were also studied in experiment with FB transplantation to rats with prior administration of lethal and sublethal doses of cyclophosphamide. In long-term cultivation of the bone marrow trephine biopsy samples, enhanced proliferation of the fibrous tissue and elimination of hemopoietic elements were seen. Combined cultivation of the trephine biopsy samples of the bone marrow and FB inhibited the fibrous tissue proliferation and enhanced the viability of the hemopoietic and lipid cells. Transplantation of the FB in experiment shortened the leukocytopenia period in rats after sublethal (300 mg/kg) and lethal (500 mg/kg) doses of cyclophosphamide and increased their survival.
在使用Marbrook系统初步培养1 - 5周后，通过骨髓环钻活检样本的形态计量分析，研究了人胎儿骨（FB）对造血细胞和基质细胞的影响。在对预先给予致死和亚致死剂量环磷酰胺的大鼠进行FB移植的实验中，也研究了大鼠FB对造血和存活的影响。在骨髓环钻活检样本的长期培养中，可见纤维组织增殖增强和造血成分消失。骨髓环钻活检样本与FB的联合培养抑制了纤维组织增殖，并提高了造血细胞和脂质细胞的活力。在实验中，FB移植缩短了给予亚致死剂量（300 mg/kg）和致死剂量（500 mg/kg）环磷酰胺后大鼠的白细胞减少期，并提高了它们的存活率。
