School of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Korea.
Bioprocess Biosyst Eng. 2012 Nov;35(9):1497-503. doi: 10.1007/s00449-012-0739-8. Epub 2012 May 30.
Oil palm fronds are the most abundant lignocellulosic biomass in Malaysia. In this study, fronds were tested as the potential renewable biomass for ethanol production. The soaking in aqueous ammonia pretreatment was applied, and the fermentability of pretreated fronds was evaluated using simultaneous saccharification and fermentation. The optimal pretreatment conditions were 7 % (w/w) ammonia, 80 °C, 20 h of pretreatment, and 1:12 S/L ratio, where the enzymatic digestibility was 41.4 % with cellulase of 60 FPU/g-glucan. When increasing the cellulase loading in the hydrolysis of pretreated fronds, the enzymatic digestibility increased until the enzyme loading reached 60 FPU/g-glucan. With 3 % glucan loading in the SSF of pretreated fronds, the ethanol concentration and yield based on the theoretical maximum after 12 and 48 h of the SSF were 7.5 and 9.7 g/L and 43.8 and 56.8 %, respectively. The ethanol productivities found at 12 and 24 h from pretreated fronds were 0.62 and 0.36 g/L/h, respectively.
油棕叶片是马来西亚最丰富的木质纤维素生物质。在本研究中，叶片被测试为生产乙醇的潜在可再生生物质。采用氨水溶液预处理，通过同步糖化发酵评价预处理叶片的发酵性能。最佳预处理条件为 7%（w/w）氨、80°C、20 h 预处理和 1:12 S/L 比，在此条件下，纤维素酶用量为 60 FPU/g-葡聚糖时，酶解率为 41.4%。当水解预处理叶片中的纤维素酶用量增加时，酶解率增加，直到纤维素酶用量达到 60 FPU/g-葡聚糖。在预处理叶片的同步糖化发酵中，当葡萄糖含量为 3%时，12 和 48 h 后基于理论最大产量的乙醇浓度和产率分别为 7.5 和 9.7 g/L 和 43.8 和 56.8%。从预处理叶片中在 12 和 24 h 时获得的乙醇生产率分别为 0.62 和 0.36 g/L/h。
